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As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.
List of contents
Cell-Based Microarrays: Overview.- Cell-Based Co-Transfection Microarrays for Use with HEK293T Cells on a Poly D-Lysine Polystyrene Coated Microplate.- Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays.- A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays.- High-Throughput Subcellular Protein Localization Using Transfected-Cell Arrays.- Cell Arrays for the Measurement of Organelle Dynamics in Living Cells.- High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays.- Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA).- Reverse Transfected Cell Microarrays in Infectious Disease Research.- Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies.- Blood Cell Capture on Antibody Micro-Arrays and Monitoring of the Cell Capture Using Surface Plasmon Resonance Imaging.- Immobilized Culture and Transfection Microarray of Nonadherent Cells.- Plasma Polymer and PEG-Based Coatings for DNA, Protein, and Cell Microarrays.- Polymer Microarrays for Cellular High-Content Screening.- High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation.- Microfluidic Image Cytometry.
Summary
As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins.
