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This second edition volume expands on the first edition by providing up-to-date protocols to characterize nanomaterials used as drug delivery agents. The chapters in this book are divided into 5 parts and cover topics such as: advances and obstacles in nanomedicine research; methods to test sterility and endotoxin, physicochemical features, immunological effects, drug release, and in vivo efficacy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Characterization of Nanoparticles Intended for Drug Delivery, Second Edition is a valuable tool for researchers and pharmaceutical and biotechnology developers who are evaluating the clinical potential of nanomedicines in preclinical studies.
List of contents
Evaluating Nanomedicines: Obstacles and Advancements.- Detection of Bacterial Contamination in Nanoparticle Formulations by Agar Plate Test.- Considerations and Some Practical Solutions to Overcome Nanoparticle Interference with LAL Assays and to Avoid Endotoxin Contamination in Nanoformulations.- Elemental Analysis in Biological Matrices Using ICP-MS.- PEG Quantitation Using Reversed Phase High Performance Liquid Chromatography and Charged Aerosol Detection.- Quantitation of Surface Coating on Nanoparticles Using Thermogravimetric Analysis.- Immunoelectron Microscopy for Visualization of Nanoparticles.- Imaging of Liposomes by Transmission Electron Microscopy.- Updated Method for In Vitro Analysis of Nanoparticle Hemolytic Properties.- In Vitro Assessment of Nanoparticle Effects on Blood Coagulation.- In Vitro Analysis of Nanoparticle Effects on the Zymosan Uptake by Phagocytic Cells.- Assessing NLRP3 Inflammasome Activation by Nanoparticles.- Analysis of Complement Activation by Nanoparticles.- Methods for Analysis of Nanoparticle Immunosuppressive Properties In Vitro and In Vivo.- Analysis of Pro-Inflammatory Cytokine and Type II Interferon Induction by Nanoparticles.- Analysis of Nanoparticle Adjuvant Properties In Vivo.- In Vitro and In Vivo Methods for Analysis of Nanoparticle Potential to Induce Delayed Type Hypersensitivity Reactions.- Autophagy Monitoring Assay II: Imaging Autophagy Induction in LLC-PK1 Cells Using GFP-LC3 Protein Fusion Construct.- Improved Ultrafiltration Method to Measure Drug Release from Nanomedicines Utilizing a Stable Isotope Tracer.- Designing an In Vivo Efficacy Study of Nanomedicines for Preclinical Tumor Growth Inhibition.
Summary
This second edition volume expands on the first edition by providing up-to-date protocols to characterize nanomaterials used as drug delivery agents. The chapters in this book are divided into 5 parts and cover topics such as: advances and obstacles in nanomedicine research; methods to test sterility and endotoxin, physicochemical features, immunological effects, drug release, and in vivo efficacy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Characterization of Nanoparticles Intended for Drug Delivery, Second Edition is a valuable tool for researchers and pharmaceutical and biotechnology developers who are evaluating the clinical potential of nanomedicines in preclinical studies.>
