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Informationen zum Autor William Hewitt studied pharmacy at the University of Nottingham. Later he studied chemistry at the University of London. He worked in the pharmaceutical industry for several years as a quality control analyst; this included the antibiotic sector, where he was introduced to and became fascinated by the microbiological potency testing of antibiotics. This was followed by work overseas as an advisor in quality control of pharmaceuticals. It was overseas that William recognized that the quantitative/mathematical principles of microbiological assay were not well understood. He prepared notes for use in his own laboratory explaining these principles. These were then expanded substantially and published in 1977 by Academic Press as: Microbiological Assay: An Introduction to Quantitative Principles and Evaluation. Few books have been written on the subject of microbiological assay, and the need was perceived for a book with more emphasis on the practical and microbiological aspects of the subject. A second book was produced in 1989, written in collaboration with Stephen Vincent, whose meticulous approach to practical detail and staff training made him an ideal partner. Theory and Application of Microbiological Assay was published by Academic Press. William has been active in promoting the understanding of microbiological assay through technology transfer in several Asian countries. He has also directed several short training courses in the U.K. and the U.S. and has acted as consultant to laboratories in Australia, Belgium, Ireland, Slovenia, and the U.K. Now retired from active laboratory work, William continues to write and produce software for assay evaluation, Hewitt Bioassays. He lives in a delightful rural part of West Wales with his wife and three cats. He has a passion for traditional jazz and plays clarinet. Klappentext A user-friendly guide for the evaluation of microbiological assays! this book provides a lucid explanation of the sources of error in microbiological assay and helps analysts choose efficient assay designs that will minimize those sources of error. The author discusses microbiological assay as a branch of pharmaceutical analysis and distinguishes it from biological assay in general. He draws attention to the microbiological aspects that may not be so obvious to the chemical analyst and to the analytical aspects that may not be so obvious to the microbiologist. The book expands on the guidance given in pharmacopoeias and helps readers choose the assay design most appropriate for the purpose of their assay. Zusammenfassung Offers an explanation of the sources of error in microbiological assay and helps analysts choose efficient assay designs that minimize those sources of error. This guide discusses microbiological assay as a branch of pharmaceutical analysis and distinguishes it from biological assay in general. Inhaltsverzeichnis MICROBIOLOGICAL ASSAY IN PERSPECTIVEBiological Assays in GeneralThe Different Sorts of Microbiological AssayThe Basis of Calculation of Potency EstimatesWhich Mathematical Model?THE AGAR DIFFUSION ASSAY - ITS QUANTITATIVE BASISHistorical IntroductionThe Theory of Zone FormationPrinciple of Calculation of Potency EstimateTHE THEORY AND PRACTICE OF TUBE ASSAYS FOR GROWTH PROMOTING SUBSTANCESIntroductionThe Mode of ActionCritical Factors in the Assay of Growth-Promoting SubstancesOther Sources of ErrorTHE THEORY AND PRACTICE OF TUBE ASSAYS FOR GROWTH INHIBITING SUBSTANCESHistorical IntroductionMeasurement of ResponseThe Form of Response LineHistorical Development of the Turbidimetric MethodLinearization of Sigmoid CurvesThe Quantitative Theory of Microbial InhibitionA Practically Determined Log Dose-Response CurveFactors Affecting Final Cell CountSummary and ConclusionsWHAT DO WE WANT OF AN ASSAY? HOW DO WE ATTAIN OUR GOAL?IntroductionPharmacopoeial IntentionControl of Antibiotic Bulk MaterialsControl in Routine M...
