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This volume covers a wide range of methods to measure cellular respiration and internal oxygen in various tissues under different conditions. Chapters guide readers through informative experimental approaches, calorespirometry, isotope fractionation techniques, protocols for dual-inlet isotope ratio mass spectrometry, laser-capture microdissection, and bioinformatics approach for exploring the co-regulation of AOX gene family members. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Respiration and Internal Oxygen: Methods and Protocols aims to be helpful for all students and researchers interested in the determination of respiration and internal oxygen.
List of contents
Assessing Metabolic Flux in Plants with Radiorespirometry.- Coupling Radiotracer Experiments with Chemical Fractionation for the Estimation of Respiratory Fluxes.- A method for Imaging Oxygen Distribution and Respiration at a Microscopic Level of Resolution.- VisiSens Technique to Measure Internal Oxygen and Respiration in Barley Roots.- MultiSense: A Multimodal Sensor Tool Enabling the High Throughput Analysis of Respiration.- Measurement of Respiration and Internal Oxygen in Germinating Cicer arietinum L. Seeds Using Micro Sensor.- Using an Oxygen Microsensor to Measure Oxygen Dynamics in Tomato Plants in Response to Pseudomonas syringe Infection.- Measurement of Oxygen Status in Arabidopsis Leaves Undergoing Hypersensitive Response During Pseudomonas Infection.- Isolation of Physiologically Active and Intact Mitochondria from Chickpea.- Isolation and Structural Studies of Mitochondria from Pea Roots.- Mitochondrial Respiration and Oxygen Tension.- Isolation of Mitochondria from Model and Crop Plants.- Procedures of Mitochondria Purification and Gene Expression to Study Alternative Respiratory and Uncoupling Pathways in Fruits.- Measurement of Tricarboxylic Acid Cycle Enzyme Activities in Plants.- Respiration Traits as Novel Markers for Plant Robustness under the Threat of Climate.- Calorespirometry - A Novel Tool in Functional Hologenomics to Select 'Green' Holobionts for Biomass Production.- Measurements of Electron Partitioning Between Cytochrome and Alternative Oxidase Pathways in Plant Tissues.- A Driving Bioinformatics Approach to Explore Co-regulation of AOX Gene Family Members during Growth and Development.- A Step-by-Step Protocol for Classifying AOX Proteins in Flowering Plants.- Studying Individual Plant AOX Gene Functionality in Early Growth Regulation - A New Approach.- Laser-Capture Microdissection for Amplification of Alternative Oxidase (AOX) Genes in Target Tissues in Daucus carota L..- Measurement of Mitochondrial Respiration in Isolated Protoplasts: Cytochrome and Alternative Pathways.- Measuring Spatial and Temporal Oxygen Flux near Plant Tissues using a Self-Referencing Optrode.
Summary
This volume covers a wide range of methods to measure cellular respiration and internal oxygen in various tissues under different conditions. Chapters guide readers through informative experimental approaches, calorespirometry, isotope fractionation techniques, protocols for dual-inlet isotope ratio mass spectrometry, laser-capture microdissection, and bioinformatics approach for exploring the co-regulation of AOX gene family members. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Respiration and Internal Oxygen: Methods and Protocols aims to be helpful for all students and researchers interested in the determination of respiration and internal oxygen.
