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This detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis. The content included addresses assays to analyze skeletal muscle gene expression, proliferating muscle cells, the process of myoblast fusion, muscle development (in vivo), as well as muscle repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Skeletal Muscle Development serves as an ideal guide to the study of this highly complex yet carefully regulated process.>
List of contents
Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells.- Whole Genome Chromatin IP-Sequencing (ChIP-Seq) in Skeletal Muscle Cells.- Analysis of RNA Expression in Adult Zebrafish Skeletal Muscle.- Chick Somite Electroporation for Gene Function Studies.- Targeted Lipidomic Analysis of Myoblasts by GC-MS and LC-MS/MS.- Measuring Mitochondrial Substrate Utilization in Skeletal Muscle Stem Cells.- Microcontact-Printed Hydrogel Microwell Arrays for Clonal Muscle Stem Cell Cultures.- Isolation, Culture, and Differentiation of Fibro/Adipogenic Progenitors (FAPs) from Skeletal Muscle.- Human Satellite Cell Isolation and Xenotransplantation.- Application of Split-GFP Reassembly Assay to Study Myogenesis and Myofusion In Vitro.- Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells.- Fabrication of Micromolded Gelatin Hydrogels for Long-Term Culture of Aligned Skeletal Myotubes.- Quantification of Embryonic Myofiber Development by Immunofluorescence.- How to Wire the Diaphragm: Wholemount Staining Methods to Analyze Mammalian Respiratory Innervation.- Membrane Repair Assay for Human Skeletal Muscle Cells.- Cryoinjury Model for Tissue Injury and Repair in Bioengineered Human Striated Muscle.
Summary
This detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis. The content included addresses assays to analyze skeletal muscle gene expression, proliferating muscle cells, the process of myoblast fusion, muscle development (in vivo), as well as muscle repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Skeletal Muscle Development serves as an ideal guide to the study of this highly complex yet carefully regulated process.>
