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This volume presents a collection of tools currently used for the characterization of rust, the host plant wheat, and their interactions. This book is divided into five parts: Parts I and II discuss advanced techniques for characterizing rust pathogens in rust surveillance, genotyping, and molecular pathogenicity; Part III describes protocols for genetic analysis of rust resistance; Part IV covers methods on rust resistance gene cloning; and Part V talks about the isolation and screening of bacterial endophytes as biocontrol agents for rust disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Wheat Rust Disease: Methods and Protocols is a valuable resource for both established and novel wheat rust researchers and also the plant science and microbial research community.
List of contents
Wheat Rust Surveillance: Field Disease Scoring and Sample Collection for Phenotyping and Molecular Genotyping.- Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance.- Race Typing of Puccinia striiformis on Wheat.- Assessment of Aggressiveness of Puccinina striiformis on Wheat.- Extraction of High Molecular Weight DNA from Fungal Rust Spores for Long Read Sequencing.- Microsatellite Genotyping of the Wheat Yellow Rust Pathogen Puccinia striiformis.- Computational Methods for Predicting Effectors in Rust Pathogens.- Protein-Protein Interaction Assays with Effector-GFP Fusions in Nicotiana benthamiana.- Proteome Profiling by 2D-Liquid Chromatography for Wheat-Rust Interaction.- Investigating Gene Function in Cereal Rust Fungi by Plant-Mediated Virus-Induced Gene Silencing.- Apoplastic Sugar Extraction and Quantification from Wheat Leaves Infected with Biotrophic Fungi.- Genetic Analysis of Resistance to Wheat Rusts.- Advances in Identificationand Mapping of Rust Resistance Genes in Wheat.- Chromosome Engineering Techniques for Targeted Introgression of Rust Resistance from Wild Wheat Relatives.- Application of Genomic Selection in Breeding Wheat for Rust Resistance.- Rapid Phenotyping Adult Plant Resistance to Stem Rust in Wheat Grown Under Controlled Conditions.- Generation of Loss of Function Mutants for Wheat Rust Disease Resistance Gene Cloning.- Isolation of Wheat Genomic DNA for Gene Mapping and Cloning.- MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes.- Rapid Gene Isolation using MutChromSeq.- Rapid Identification of Rust Resistance Genes through Cultivar-Specific de novo Chromosome Assemblies.- BSMV-Induced Gene Silencing Assay for Functional Analysis of Wheat Rust Resistance.- Yeast as a Heterologous System to Functionally Characterize a Multiple Rust Resistance Gene which Encodes a Hexose Transporter.- Biocontrol Agents for Controlling Wheat Rust.
Summary
This volume presents a collection of tools currently used for the characterization of rust, the host plant wheat, and their interactions. This book is divided into five parts: Parts I and II discuss advanced techniques for characterizing rust pathogens in rust surveillance, genotyping, and molecular pathogenicity; Part III describes protocols for genetic analysis of rust resistance; Part IV covers methods on rust resistance gene cloning; and Part V talks about the isolation and screening of bacterial endophytes as biocontrol agents for rust disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Wheat Rust Disease: Methods and Protocols is a valuable resource for both established and novel wheat rust researchers and also the plant science and microbial research community.
