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Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, and bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.
List of contents
Foreword Keith Wilson and John Walker; Preface Samuel Clokie and Andreas Hofmann; Tables and resources Andreas Hofmann; 1. Life science studies Samuel Clokie and Andreas Hofmann; 2. Basic principles Parisa Amani and Andreas Hofmann; 3. Cell culture techniques Anwar Baydoun; 4. Recombinant DNA techniques and molecular cloning Ralph Rapley; 5. Preparative biochemistry Samuel Clokie; 6. Electrophoresis Ralph Rapley; 7. Immunochemical techniques Katja Fischer; 8. Flow cytometry John Grainger and Joanne Konkel; 9. Radioisotope techniques Robert Slater; 10. Clinical biochemistry Gillian Rumsby; 11. Microscopy Stephen Paddock; 12. Centrifugation Kay Ohlendieck and Stephen Harding; 13. Spectroscopic techniques Anne Simon and Andreas Hofmann; 14. Basic techniques probing molecular structure and interactions Anne Simon and Joanne Macdonald; 15. Mass spectrometric techniques Sonja Hess and James MacRae; 16. Fundamentals of bioinformatics Cinzia Cantacessi and Anna Protasio; 17. Fundamentals of cheminformatics Paul Taylor; 18. The Python programming language Timothy Stevens; 19. Processing and visualisation of experimental data Jean-Baptiste Cazier; 20. Fundamental genome sequencing and annotation Pasi Korhonen and Robin Gasser; 21. Fundamentals of proteomics Sonja Hess and Michael Weiss; 22. Fundamentals of metabolomics James MacRae; 23. Enzymes and receptors Megan Cross and Andreas Hofmann; 24. Drug discovery and development David Camp.
Summary
This best-selling undergraduate textbook introduces the key experimental techniques from across the biosciences. It uniquely integrates the theories and methods driving the fields of biology and medicine, comprehensively covering both the techniques that students will encounter in lab classes and those that underpin recent advances and discoveries.
Report
'After seven editions between 1975 and 2010 this essential textbook has undergone a major update. The new editors, Drs Hofmann and Clokie, recruited many experts to revise individual chapters and added a significant amount of new material, thus making the book even more valuable. This eighth edition is basically a new book that covers the techniques of biochemistry and molecular biology in a very comprehensive manner - it does not go into great detail, but gives the reader a good introduction to all of them. Each chapter contains suggestions for further reading for those in need of a follow-up. Excellent diagrams and illustrations provide help in comprehension of the presented material. Since this text is largely aimed at undergraduate students, it fulfils its role extremely well.' Alexander Wlodawer, National Cancer Institute at Frederick
