Viral Applications of Green Fluorescent Protein - Methods and Protocols

Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology(TM) series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.

List of contents

Using Fluorescent Proteins to Study Poxvirus Morphogenesis.- Molecular Imaging of Human Embryonic Stem Cells.- Use of a GFP-PML-Expressing Cell Line as a Biosensor for Human Cytomegalovirus Infection.- Dual-Color Imaging of Tumor Angiogenesis.- Use of GFP to Analyze Morphology, Connectivity, and Function of Cells in the Central Nervous System.- Expression of EGFP by Adenovirus-Mediated Gene Transfer in the Central Nervous System.- Adenovirus-Mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac Myocytes.- Microscopic Analysis of Adenoviral Decontamination Using GFP Adenovirus with Comparable Sensitivity to Flow Cytometry.- Production of Multicistronic HIV-1 Based Lentiviral Vectors.- Using an EGFPmeter to Evaluate the Lentiviral Vector Production: Tricks and Traps.- Use of a Macrophage-Tropic GFP-Tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral Reservoirs.- GFP-Lentiviral Vectors Targeting for NeuroAIDS.- Construction of Cell-Type Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene Delivery.- Selective Protein Expression Within the CNS Using Hybrid Lentivirus.- Construction and Analysis of Alphaherpesviruses Expressing Green Fluorescent Protein.- The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication.- Modified Bovine Herpesvirus 1 for Protein Secretion.- Identification of Viral Peptide Fragments for Vaccine Development.- Use of GFP to Investigate Expression of Plant-Derived Vaccines.- Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions.- Sendai Virus for Cancer Immunotherapy.- Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic Cells.- Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity.- Baculovirus-Mediated Gene Transfer into Mesenchymal Stem Cells.

Summary

Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology™ series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.

Additional text

From the reviews:
"One of the most useful modifications that have been made to viruses is the ability to encode tracers such as green fluorescent protein (GFP) … thereby allowing not only study of the virus itself, but also the behaviour of infected cells. As the title indicates, it is a methods text, giving useful practical details important to those attempting to carry out similar studies." (Christopher Ring, Microbiology Today, November, 2009)
"This volume in the Methods in Molecular Biology series is a compilation of laboratory protocols organized into 24 chapters dealing with the diverse viral delivery systems for green fluorescent proteins. … The book is written primarily for practicing scientists involved in the study of green fluorescent expression in live cells and experimental animals. … provides a number of updated protocols for the practicing investigator. … a valuable companion for all researchers involved in the use of green fluorescent proteins in biological systems." (Ruben Mestril, Doody’s Review Service, September, 2009)
“This book focuses on a very important area of application of green fluorescent protein (GFP) and its variants … . The significance of viral applications of fluorescent proteins is well covered … . it will certainly be a valuable source of information and a good basis for laboratory protocols for researchers from all different fields of life sciences.” (Johannes Schmid, ChemBioChem, Vol. 10, 2009)
“Cover the use of Green Fluorescent Protein (GFP) technology to study aspects of the replication of viruses and the majority of the chapters describe the use of viruses and GFP as research tools in a much wider context. … I enjoyed the book for the breadth of topics and snapshots of research … . a more likely home for this book is a university or institute library where its eclectic contents will have a sufficiently wide audience.” (Paul Monaghan, Infocus Magazine, Issue 19,September, 2010)

Report

From the reviews:
"One of the most useful modifications that have been made to viruses is the ability to encode tracers such as green fluorescent protein (GFP) ... thereby allowing not only study of the virus itself, but also the behaviour of infected cells. As the title indicates, it is a methods text, giving useful practical details important to those attempting to carry out similar studies." (Christopher Ring, Microbiology Today, November, 2009)
"This volume in the Methods in Molecular Biology series is a compilation of laboratory protocols organized into 24 chapters dealing with the diverse viral delivery systems for green fluorescent proteins. ... The book is written primarily for practicing scientists involved in the study of green fluorescent expression in live cells and experimental animals. ... provides a number of updated protocols for the practicing investigator. ... a valuable companion for all researchers involved in the use of green fluorescent proteins in biological systems." (Ruben Mestril, Doody's Review Service, September, 2009)
"This book focuses on a very important area of application of green fluorescent protein (GFP) and its variants ... . The significance of viral applications of fluorescent proteins is well covered ... . it will certainly be a valuable source of information and a good basis for laboratory protocols for researchers from all different fields of life sciences." (Johannes Schmid, ChemBioChem, Vol. 10, 2009)
"Cover the use of Green Fluorescent Protein (GFP) technology to study aspects of the replication of viruses and the majority of the chapters describe the use of viruses and GFP as research tools in a much wider context. ... I enjoyed the book for the breadth of topics and snapshots of research ... . a more likely home for this book is a university or institute library where its eclectic contents will have a sufficiently wide audience." (Paul Monaghan, Infocus Magazine, Issue 19,September, 2010)

Product details

• Assisted by: Barry W. Hicks (Editor), Barr W Hicks (Editor), Barry W Hicks (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 01.01.2017
• EAN: 9781493961436
• ISBN: 978-1-4939-6143-6
• No. of pages: 357
• Dimensions: 193 mm x 20 mm x 260 mm
• Weight: 776 g
• Illustrations: XI, 357 p. 81 illus., 35 illus. in color.
• Series: Methods in Molecular Biology; Methods in Molecular Biology
• Subjects: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics; B, Medical research, Life Sciences, proteins, Biomedical and Life Sciences, Biomedical Research, Medical laboratory testing & techniques, Laboratory Medicine, Protein Science