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Parasite Genomics Protocols, Second Edition expands upon the previous edition with current, detailed methodologies that have been adapted to research parasites in the post genomic era. With new chapters on parasite studies, helminth pathogens, and protocols for undertaking other large-scale 'omics' methodologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Parasite Genomics Protocols, Second Edition offers a wide-ranging collection of diverse methods and protocols for further study into these unique organisms.
List of contents
The Eukaryotic Pathogen Databases: A Functional Genomic Resource Integrating Data from of Human and Veterinary Parasites.- From Sequence Mapping to Genome Assemblies.- Sequencing and Annotation of Mitochondrial Genomes from Individual Parasitic Helminths.- A Beginners Guide to Estimating the Non-synonymous to Synonymous Rate Ratio of all Protein-coding Genes in a Genome.- Exploiting Genetic Variation to Discover Genes Involved in Important Disease Phenotypes.- Identification and Analysis of Ingi-Related Retroposons in the Trypanosomatid Genomes.- Approaches for Studying mRNA Decay Mediated by SIDER2 Retroposons in Leishmania.- Gene Suppression in Schistosomes using RNAi.- Construction of Trypanosoma brucei Illumina RNA-Seq Libraries Enriched for Transcript Ends.- Techniques to Study Epigenetic Control and the Epigenome in Parasites.- The Genome-wide Identification of Promoter Regions in Toxoplasma gondii.- RNA-seq Approaches for Determining mRNA Abundance in Leishmania.- Protein Microarrays for Parasite Antigen Discovery.- A Transposon-based Tool for Transformation and Mutagenesis in Trypanosomatid Protozoa.- Separation of Basic Proteins from Leishmania using a combination of Free Flow Electrophoresis (FFE) and 2D Electrophoresis (2-DE) under Basic Conditions.- Proteomic Analysis of Post Translational Modifications using iTRAQ in Leishmania.- Large Scale Differential Proteome Analysis in Plasmodium falciparum under Drug Treatment.- Use of 13C Stable Isotope Labelling for Pathway and Metabolic Flux Analysis in Leishmania Parasites.- Molecular Genotyping of Trypanosoma cruzi for Lineage Assignment and Population Genetics.- Screening Leishmania donovani Complex-specific Genes Required for Visceral Disease.
Summary
Parasite Genomics Protocols, Second Edition expands upon the previous edition with current, detailed methodologies that have been adapted to research parasites in the post genomic era. With new chapters on parasite studies, helminth pathogens, and protocols for undertaking other large-scale ‘omics’ methodologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Parasite Genomics Protocols, Second Edition offers a wide-ranging collection of diverse methods and protocols for further study into these unique organisms.
