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This second edition provides up-to-date and new chapters covering the most important widely-used techniques in plant stress tolerance, including cutting-edge strategies, in a manner that ensures effective results. Plant Stress Tolerance: Methods and Protocols, Second Edition guides readers through reviews on epigenetics, long noncoding RNAs, and the influence of microbiome on drought tolerance, the volume then continues with methods describing the identification of stress-responsive genes, proteins, posttranslational redox modifications, DNA methylation, histone modifications, measurement of glutathione, lipids, raffinose family oligosaccharides, polyamines, as well as quantification of abscisic acid, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Stress Tolerance: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
List of contents
Epigenetics and RNA Processing: Connections to Drought, Salt and ABA?.- The Fundamental Role Of Reactive Oxygen Species In Stress Response.- Role of Long Non-coding RNAs on Plant Stress Tolerance.- Towards a Resilient, Functional Microbiome: Drought Tolerance-Alleviating Microbes for Sustainable Agriculture.- Mining and Quantifying In Vivo Molecular Interactions in Abiotic Stress Acclimation.- Generation of a Stress-inducible Luminescent Arabidopsis and Its Use in Genetic Screening for Stress-responsive Gene Deregulation Mutants.- Detection of Differential DNA Methylation under Stress Conditions using Bisulfite-Sequence Analysis.- ChIP-seq Analysis for Identifying Genome-wide Histone Modifications Associated With Stress-responsive Genes in Plants .- Isolation of Polysomal RNA for Analyzing Stress-Responsive Genes Regulated at the Translational Level in Plants.- Global Proteomic Profiling and Identification of Stress-Responsive Proteins using Two-dimensional Gel Electrophoresis.- Phosphoproteomics Analysis for Probing Plant Stress Tolerance.- Probing Posttranslational Redox Modifications.- Zymographic Method for Distinguishing Different Classes of Superoxide Dismutases in Plants.- Determination of Enzymes Associated with Sulfite Toxicity in Plants: Kinetic Assays For SO, APR, SiR and in-gel SiR Activity.- Determination of Total Sulfur, Sulfate, Sulfite, Thiosulfate, and Sulfolipids in Plants.- Determining Glutathione Levels in Plans.- Porous Graphitic Carbon Liquid Chromatography-Mass Spectrometry Analysis of Drought Stress-responsive Raffinose Family Oligosaccharides in Plant Tissues.- Profiling Abscisic Acid Induced Changes in Fatty Acid Composition in Mosses.- Detection of Free Polyamines in Plants Subjected to Abiotic Stresses by High-Performance Liquid Chromatography (HPLC).- Determination of Polyamines by Dansylation, Benzoylation, and Capillary Electrophoresis.- Rapid Quantification of Abscisic Acid by GC-MS/MS for studies of Abiotic Stress Response.- Silencing of Stress-regulated miRNAs in Plants by Short Tandem Target Mimic (STTM) Approach.- Rhizosphere Sampling Protocols for Microbiome (16S/18S/ITS rRNA) Library Preparation and Enrichment for the Isolation of Drought Tolerance-Promoting Microbes.
Summary
This second edition provides up-to-date and new chapters covering the most important widely-used techniques in plant stress tolerance, including cutting-edge strategies, in a manner that ensures effective results. Plant Stress Tolerance: Methods and Protocols, Second Edition guides readers through reviews on epigenetics, long noncoding RNAs, and the influence of microbiome on drought tolerance, the volume then continues with methods describing the identification of stress-responsive genes, proteins, posttranslational redox modifications, DNA methylation, histone modifications, measurement of glutathione, lipids, raffinose family oligosaccharides, polyamines, as well as quantification of abscisic acid, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Stress Tolerance: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
