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This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Circula
ting Tumor Cells: Methods and Protocols is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs.
List of contents
Microfluidic Capture and Multiplex Immunofluorescence of Circulating Tumor Cells to Identify Cancer Origin.- Microfluidic Separation of Circulating Tumor Cells Cased on Size and Deformability.- A Novel Microfluidic Device for Isolation of Circulating Tumor Cells from Pancreatic Cancer Blood Samples.- Microfluidic-Based Enrichment and Retrieval of Circulating Tumor Cells for RT-PCR Analysis.- Micro-Scale Laminar Vortices for high-Purity Extraction and Release of Circulating Tumor Cells.- Separable Bilayer Microfiltration Device for Viable Label-Free Enrichment of Circulating Tumor Cells.- Microfilter-Based Capture and Release of Viable Circulating Tumor Cells.- Fourier Ptychographic Microscopy for Rapid, High Resolution Imaging of Circulating Tumor Cells Enriched by Microfiltration.- Automated Microfluidic Filtration and Immunocytochemistry Detection System for Capture and Enumeration of Circulating Tumor Cells and Other Rare Cell Populations in Blood.- Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells.- Negative Enrichment and Isolation of Circulating Tumor Cells for Whole Genome Amplification.- Capture and Genetic Analysis of Circulating Tumor Cells Using a Magnetic Separation Device (Magnetic Sifter).- RareCyte®CTC Analysis Step 1: AccuCyte® Sample Preparation for the Comprehensive Recovery of Nucleated Cells from Whole Blood.- RareCyte®CTC Analysis Step 2: Detection of Circulating Tumor Cells by Cytefinder® Automated Scanning and Semi-Automated Image Analysis.- RareCyte®CTC Analysis Step 3: Using Cytepicker® Module for Individual Cell Retrieval and Subsequent Whole Genome Amplification of Circulating Tumor Cells for Genomic Analysis.- Enumeration, Dielectrophoretic Capture, and Molecular Analysis of Circulating Tumor Cells.- Enumeration of Circulating Tumor Cells and Disseminated Tumor Cells in Blood and Bone Marrow by Immunomagnetic Enrichment and Flow Cytometry (IE/FC).- Flow Cytometric Detection of Circulating Tumor Cells Using a Candidate Stem Cell Marker, p75 Neurotrophin Receptor (p75NTR).- Multispectral Imaging Analysis of Circulating Tumor Cells in Negatively-Enriched Peripheral Blood Samples.- Fiber-Optic Array Scanning Technology (FAST) for Detection and Molecular Characterization of Circulating Tumor Cells.- A Non-Invasive and Real-Time Method for Circulating Tumor Cell Detection: In Vivo Flow Cytometry.- EpCAM-Independent Enrichment and Detection of Viable CTCs Using the EPISPOT Assay.- Utilizing Matrigel Transwell Invasion Assay to Detect and Enumerate Circulating Tumor Cells.- Circulating Tumor Cells: Markers and Methodologies for Enrichment and Detection.
Summary
This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Circula
ting Tumor Cells: Methods and Protocols is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs.
