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This second edition provides an update on technology and an accelerated tutorial to assist, students, entrepreneurs, new investigators, and established investigators who want to quickly become versed in, and immersed in, the entire process from discovery to clinical trial validation and commercial public benefit. Chapters aim to cover the full spectrum of molecular profiling from tumor staging and grading through biomarker discovery, to commercialization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Molecular Profiling: Methods and Protocols, Second Edition aims to be a useful guide on molecular profiling.
List of contents
Tumor Staging and Grading: A Primer.- Innovations in Clinical Trial Design in the Era of Molecular Profiling.- Personalized Medicine: Ethical Aspects.- Antibody Validation by Western Blotting.- Scanning Electron Microscopy Sample Preparation and Imaging.- One Step Preservation and Decalcification of Bony Tissue for Molecular Profiling.- Application of Hydrogel Nanoparticles for the Capture, Concentration, and Preservation of Low-Abundance Biomarkers.- Using Laser Capture Microdissection to Isolate Cortical Laminae in Non-human Primate Brain.- Western Blot Techniques.- ELISA for Monitoring Nerve Growth Factor.- Reverse Phase Protein Microarrays.- Clustering and Network Analysis of Reverse-Phase Protein Array Data.- PCR: Identification of Genetic Polymorphisms.- Microsatellite Analysis for Identification of Individuals Using Bone from the Extinct Steller's Sea Cow (Hydrodamalis gigas).- Somatic DNA Mutation Analysis.- Optimization of Immunostaining for Prospective Image Analysis.- Fluorescence in situ Hybridization of Cells, Chromosomes, and Formalin Fixed Paraffin Embedded Tissues.- High-Resolution Image Stitching as a Tool to Assess Tissue-Level Protein Distribution and Localization.- Mass Spectrometry Based Biomarker Discovery.- Quantitative Mass Spectrometry by Isotope-Dilution and Multiple Reaction Monitoring (MRM).- LC-Mass Spectrometry for Metabolomics.- Metabolomic Bioinformatic Analysis.- Stable Isotope Quantitative N-glycan Analysis by Liquid Separation Techniques and Mass Spectrometry.- Grant Writing Tips for Translational Research.- Inventions and Patents: A Practical Tutorial.- Product Development and Commercialization of Diagnostic or Life Science Products for Scientists and Researchers.
Summary
This second edition provides an update on technology and an accelerated tutorial to assist, students, entrepreneurs, new investigators, and established investigators who want to quickly become versed in, and immersed in, the entire process from discovery to clinical trial validation and commercial public benefit. Chapters aim to cover the full spectrum of molecular profiling from tumor staging and grading through biomarker discovery, to commercialization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Molecular Profiling: Methods and Protocols, Second Edition aims to be a useful guide on molecular profiling.
