Biofilms

Volume 310 of Methods in Enzymology is the first volume devoted solely to biofilm research methods. It provides a contemporary source book for virtually any kind of experimental approach involving biofilms. It includes bioengineering, molecular, genetic, microscopic, chemical, continuous culture, and physical methods. This volume will serve as a starting point for future developments.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

List of contents

Contributors to Volume 310.
Preface.
Volumes in Series.
Molecular Biology of Biofilm Bacteria:
D.R. Korber, G.M. Wolfaardt, V. Brozel, R. MacDonald, and T. Niepel, Reporter Systems for Microscopic Analysis of Microbial Biofilms.
S. Molin, B.B. Christensen, C. Sternberg, J.B. Andersen, R.J. Palmer, Jr., A.T. Nielsen, and M. Giskov, Molecular Tools for the Study of Biofilm Physiology.
E.P. Greenberg and M.R. Parsek, Quorum Sensing Signals in the Development of Pseudomonas aeruginosa Biofilms.
C. Prignet-Combaret and P. Lejeune, Monitoring Gene Expression in Biofilms.
W. Manz, In Situ Analysis of Microbial Biofilms by rRNA-Targeted Oligonucleotide Probing.
G.A. O'Toole, L.A. Pratt, P.I. Watnick, D.K. Newman, V.B. Weaver, and R. Kolter, Genetic Approaches to the Study of Biofilms.
T.R. De Kievit and B.H. Iglewski, Quorum Sensing, Gene Expression, and Pseudomonas Biofilms.
Microscopic Methods of Biofilm Formation and Physiology:
J.R. Lawrence and T.R. Neu, Confocal Laser Scanning Microscopy for Analysis of Microbial Biofilms.
T.R. Neu and J.R. Lawrence, Lectin Binding Analysis in Biofilm Systems.
R.J. Palmer, Jr. and D.C. White, Spatially Resolved, Quantitative Determination of Luciferase Activity by Photon-Counting Microscopy.
R.J. Palmer, Jr. , Microscopy Flowcells: Perfusion Chambers for Real-Time Study of Biofilms.
J.T. Lisle, P.S. Stewart, and G.A. McPeters, Fluorescent Probes Applied to the Physiological Characterization of Bacterial Biofilms.
D. Phipps, G. Rodriguez, and H. Ridgway, Deconvolution Fluorescence Microscopy for Observation and Analysis of Membrane Biofilm Architecture.
T.A. Fissel and C.E. Edmiston, Jr., Bacterial Biofilms: New Strategies for Preparing Glycocalyx for Electron Microscopy.
Flow and Steady-State Methods:
A. Kharazmi, B. Giwereman, and N. Hoiby, The Robbins Device in Biofilm Research.
G.H.W. Bowden, A Controlled Environment Model for Accumulation of Biofilms of Oral Bacteria.
M.S. Zinn, R.D. Kirkegard, R.J. Palmer, Jr., and D.C. White, Laminar Flow Chamber for Continuous Monitoring of Biofilm Formation and Succession.
D. Allison and P. Gilbert, Perfused Biofilm Fermenters.
R.J.C. McLean, M. Whiteley, B.C. Hoskins, P. Majors, and M.M. Sharma, Laboratory Techniques for Studying Biofilm Growth, Physiology, and Gene Expression in Flowing Systems and Porous Media.
M. Wilson, Use of the Constant Depth Film Fermentor in Studies of Biofilms and Oral Bacteria.
D.J. Bradshaw and P.D. Marsh, The Use of Continuous Flow Techniques in Modeling Dental Plaque Biofilms.
G. Dibdin and J. Wimpenny, Steady-State Biofilm: Practice and Theoretical Models.
PE. Kolenbrander, R.N. Andersen, K. Kazmerzak, R. Wu, and R.J. Palmer, Jr. , Spatial Organization of Oral Bacteria in Biofilm.
Biofilms of Archae:
P.L. Hartzell, J. Millstein, and C. LaPaglia, Biofilm Formation in Hyperthermophilic Archaean.
Physical Methods:
C. Johansen, B.K. Bredtved, and S. Moller, Use of Conductance Measurements for Determination of Enzymatic Degradation of Microbial Biofilm.
W. Sand and H. von Rege, Evaluation and Quantification of Bacterial Attachment, Microbial Activity, and Biocide Efficacy by Microcalorimetry.
Y.F. Duferen, J.P. Boonaert, and P.G. Rouxhet, Surface Analysis by X-Ray Photoelectron Spectroscopy in the Study of Bioadhesion and Biofilms.
R.N. Jordan, Evaluating Biofilm Activity in Response to the Mass Transfer-Limited Bioavailablity of Sorbed Nutrients.
R.Weiner, E. Seagren, C. Arnosti, and E. Quintero, Bacterial Survival in Biofilms: Probes for Exopolysaccharide and its Hydrolysis, and Measurements of Intra- and Interphase Mass Fluxes.
G. Reid, C. Heinemann, M. Velraeds, a

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Product details

• Publisher: Academic Press
• Languages: English
• Product format: Hardback
• Released: 01.01.1999
• EAN: 9780121822118
• ISBN: 978-0-12-182211-8
• Weight: 1146 g
• Illustrations: w. figs., col. plates.
• Series: Methods in Enzymology; Methods in Enzymology
• Subject: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics