Science of Stem Cells

Informationen zum Autor JONATHAN M. W. SLACK is a developmental biologist and author of five books and over 200 scientific papers. He is an emeritus professor at the University of Bath, UK, as well as the University of Minnesota, USA, where he was Director of the Stem Cell Institute from 2007-2013. Klappentext Introduces all of the essential cell biology and developmental biology background for the study of stem cellsThis book gives you all the important information you need to become a stem cell scientist. It covers the characterization of cells, genetic techniques for modifying cells and organisms, tissue culture technology, transplantation immunology, properties of pluripotent and tissue specific stem cells and, in particular, the relevant aspects of mammalian developmental biology. It dispels many misconceptions about stem cells--especially that they can be miracle cells that can cure all ills. The book puts emphasis on stem cell behavior in its biological context and on how to study it. Throughout, the approach is simple, direct, and logical, and evidence is given to support conclusions.Stem cell biology has huge potential for advancing therapies for many distressing and recalcitrant diseases, and its potential will be realized most quickly when as many people as possible have a good grounding in the science of stem cells.* Content focused on the basic science underpinning stem cell biology* Covers techniques of studying cell properties and cell lineage in vivo and in vitro* Explains the basics of embryonic development and cell differentiation, as well as the essential cell biology processes of signaling, gene expression, and cell division* Includes instructor resources such as further reading and figures for downloading* Offers an online supplement summarizing current clinical applications of stem cellsWritten by a prominent leader in the field, The Science of Stem Cells is an ideal course book for advanced undergraduates or graduate students studying stem cell biology, regenerative medicine, tissue engineering, and other topics of science and biology. Zusammenfassung Introduces all of the essential cell biology and developmental biology background for the study of stem cellsThis book gives you all the important information you need to become a stem cell scientist. It covers the characterization of cells! genetic techniques for modifying cells and organisms! tissue culture technology! transplantation immunology! properties of pluripotent and tissue specific stem cells and! in particular! the relevant aspects of mammalian developmental biology. It dispels many misconceptions about stem cells--especially that they can be miracle cells that can cure all ills. The book puts emphasis on stem cell behavior in its biological context and on how to study it. Throughout! the approach is simple! direct! and logical! and evidence is given to support conclusions.Stem cell biology has huge potential for advancing therapies for many distressing and recalcitrant diseases! and its potential will be realized most quickly when as many people as possible have a good grounding in the science of stem cells.* Content focused on the basic science underpinning stem cell biology* Covers techniques of studying cell properties and cell lineage in vivo and in vitro* Explains the basics of embryonic development and cell differentiation! as well as the essential cell biology processes of signaling! gene expression! and cell division* Includes instructor resources such as further reading and figures for downloading* Offers an online supplement summarizing current clinical applications of stem cellsWritten by a prominent leader in the field! The Science of Stem Cells is an ideal course book for advanced undergraduates or graduate students studying stem cell biology! regenerative medicine! tissue engineering! and other topics of science and biology. Inhaltsverzeichnis Pre...

List of contents

Preface xi
 
About the Companion Website xiii
 
1 What is a Stem Cell? 1
 
Stem Cell Markers 3
 
Label-Retention 4
 
The Niche 5
 
Asymmetric Division and Differentiated Progeny 6
 
Clonogenicity and Transplantation 6
 
In Vivo Lineage Labeling 7
 
Conclusions 9
 
Further Reading 10
 
2 Characterizing Cells 13
 
Histological and Anatomical Methods 13
 
Histological Sections 13
 
Fixation 13
 
Sectioning 14
 
Staining 14
 
Electron Microscopy 15
 
Fluorescence Microscopy 16
 
Wholemounts 17
 
Immunostaining 17
 
In Situ Hybridization 18
 
Other Methods 19
 
RNAseq 19
 
Laser Capture Microdissection 19
 
Flow Cytometry 20
 
Dividing Cells 21
 
The Cell Cycle 21
 
Studying Cell Turnover 24
 
Reporters for the Cell Cycle 26
 
Identification of Very Slow Cell Turnover 26
 
Classification of Cell Types by Proliferative Behavior 28
 
Cell Death 28
 
Further Reading 30
 
3 Genetic Modification and the Labeling of Cell Lineages 31
 
Introducing Genes to Cells 31
 
Transfection and Electroporation 31
 
Gene Delivery Viruses 33
 
Controlling Gene Expression 35
 
Tet System 35
 
Cre System 35
 
Inhibiting Gene Activity 37
 
CRISPR-Cas9 37
 
Transgenic Mice 38
 
Animal Procedures 38
 
Modification of Embryonic Stem Cells 40
 
Types of Transgenic Mice 41
 
Cell Lineage 42
 
Cell Lineage, Fate Maps, Clonal Analysis 43
 
Use of CreER for Lineage Analysis 44
 
Retroviral Barcoding 46
 
Clonal Analysis in Humans 47
 
Further Reading 47
 
4 Tissue Culture, Tissue Engineering and Grafting49
 
Simple Tissue Culture 51
 
Media 51
 
Contamination 53
 
Growth in Culture 54
 
Cryopreservation and Banking 55
 
GMP Cultivation 56
 
Complex Tissue Culture 56
 
Induced Differentiation 56
 
Three Dimensional Cell Culture 57
 
Artificial Organs and Organoids 59
 
Grafting 60
 
The Immune System 61
 
T Cells 61
 
The Major Histocompatibility Complex 62
 
T and B Cell Responses 63
 
Reactions to a Graft 64
 
Immunosuppressive Drugs 65
 
Animal Experiments Involving Grafting 66
 
Further Reading 67
 
5 Early Mouse and Human Development 69
 
Gametogenesis 70
 
Germ Cells 70
 
Mitosis and Meiosis 70
 
Primordial Germ Cells (PGCs) 72
 
Spermatogenesis 73
 
Oogenesis 73
 
Fertilization 76
 
Early Development 77
 
Preimplantation Phase 77
 
Implantation Period - Mouse 80
 
Implantation Period - Human 82
 
Ethical and Legal Issues Concerning the Early Human Conceptus 85
 
Sex Determination 86
 
X-Inactivation 87
 
Imprinting 87
 
Cloning by Nuclear Transplantation (SCNT) 89
 
Further Reading 90
 
6 Pluripotent Stem Cells 93
 
Mouse Pluripotent Stem Cells 93
 
Mouse Embryonic Stem Cells 93
 
Differentiation of Mouse ES cells 95
 
Mouse iPS Cells 97
 
Human Pluripotent Stem Cells 101
 
SCNT-Derived Embryonic Stem Cells 102
 
Ethical Issues Concerning Human ES Cells 102
 
Pluripotent Stem Cells from Postnatal Organisms 103
 
Applications of Pluripotent Stem Cells 104
 
Further Re

Product details

• Authors: J Slack, J. M. W. Slack, Jonathan M. W. Slack, Jonathan M. W. (University of Bath) Slack, Slack Jonathan M. W.
• Publisher: Wiley, John and Sons Ltd
• Languages: English
• Product format: Hardback
• Released: 04.01.2018
• EAN: 9781119235156
• ISBN: 978-1-119-23515-6
• No. of pages: 272
• Subjects: Natural sciences, medicine, IT, technology > Biology > General, dictionaries; Medizin, Life Sciences, Entwicklungsbiologie, ZELLBIOLOGIE, Biowissenschaften, Stammzelle, Cell Biology, Developmental biology, Medical Science, Medizinische Zellbiologie, Medical Cell Biology