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This practical volume brings together a broad range of topics related to membrane biology research with particular emphasis on novel approaches, technology platforms, and emerging tools in this area of study. Beginning with chapters pertaining to artificial or designer membrane mimetics that can be utilized for in vitro studies of membrane-lipid interactions, it then continues by covering the approaches and methodologies to directly investigate membrane protein structure, localization, and dynamics. Written for the Springer Protocols Handbooks series, this collection focuses on in-depth, hands-on protocols from experts in the field.
Authoritative and cutting-edge, Chemical and Synthetic Approaches in Membrane Biology serves as an ideal guide to researchers investigating the vital properties of cellular membranes.
List of contents
Lipid-Coated Gold Nanoparticles as Probes for Membrane Binding.- Delving into Lipid-Driven Endocytic Mechanisms Using Biomimetic Membranes.- Lipid Bilayer Patterns Fabrication by One-Photon Lithography.- Approaches for Preparation and Biophysical Characterization of Transmembrane beta-Barrels.- Probing the Dynamics of Plasma Membrane Glutamate Transporters in Real-Time by Total Internal Fluorescence Reflection Microscopy.- Fluorescence-Based Screening of Membrane Proteins for Structural Studies.- Single-Molecule Localization Super-Resolution Microscopy of Synaptic Proteins.- Chemical Methods for Monitoring Protein Fatty-Acylation.- E. coli Membrane for In Situ Magic Angle Spinning Solid-State Nuclear Magnetic Resonance Studies.
Summary
This practical volume brings together a broad range of topics related to membrane biology research with particular emphasis on novel approaches, technology platforms, and emerging tools in this area of study. Beginning with chapters pertaining to artificial or designer membrane mimetics that can be utilized for in vitro studies of membrane-lipid interactions, it then continues by covering the approaches and methodologies to directly investigate membrane protein structure, localization, and dynamics. Written for the Springer Protocols Handbooks series, this collection focuses on in-depth, hands-on protocols from experts in the field.
Authoritative and cutting-edge, Chemical and Synthetic Approaches in Membrane Biology serves as an ideal guide to researchers investigating the vital properties of cellular membranes.
