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This volume presents cutting-edge techniques to design, fabricate and use compartmentalized microfluidic devices. Chapters encompass axon guidance and manipulation, compartmentalized devices for synapse manipulation, cell populations interaction either in physiological or pathological condition and compartmentalized devices for pharmacological research and drug discovery. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.
Authoritative and practical, Microfluidic and Compartmentalized Platforms for Neurobiological Research provides practical skills needed to fabricate, use microfluidics and compartmentalized platforms with neuronal cell cultures as well as the strengths of these exciting devices and their precious contribution in the field of Neuroscience.
List of contents
The Fabrication of Microfluidic Platforms with Pneumatically/Hydraulically Controlled PDMS Valves and their use in Neurobiological Research.- A Reliable Reversible Bonding Method for Perfused Microfluidic Devices.- Bridging Two Cultures: Minimalistic Networks Prepared by Microfluidic Arraying and Open Access Compartments for Electrophysiology.- Asymmetric Genetic Manipulation and Patch Clamp Recording of Neurons in a Microfluidic Chip.- Development of a Compartmentalized Biochip for Axonal Isolation and Neuronal-Circuit Formation at the Single-Cell Level.- Campenot Cultures and Microfluidics Provide Complementary Platforms for Spatial Study of Dorsal Root Ganglia Neurons.- Development of Microfluidic Devices for the Manipulation of Neuronal Synapses.- Use of a 3 Compartment Microfluidic Device to Study Activity Dependent Synaptic Competition.- Multi-Compartment Neuron-Glia Co-Culture Microsystem.- Compartmentalized Microfluidic Platforms as Tool of Choice to Study the Interaction Between Neurons and Osteoblasts.- A Novel In Vitro Primary Culture Model of the Lower Motor Neuron-Neuromuscular Junction Circuit.- Compartmentalized Microfluidics for In Vitro Alzheimer's Disease Studies.- Selective Biochemical Manipulation of Twin Neuronal Networks on Micro Electrode.- Compartmentalized Synapse Microarray for High-Throughput Screening.
Summary
This volume presents cutting-edge techniques to design, fabricate and use compartmentalized microfluidic devices. Chapters encompass axon guidance and manipulation, compartmentalized devices for synapse manipulation, cell populations interaction either in physiological or pathological condition and compartmentalized devices for pharmacological research and drug discovery. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Authoritative and practical, Microfluidic and Compartmentalized Platforms for Neurobiological Research provides practical skills needed to fabricate, use microfluidics and compartmentalized platforms with neuronal cell cultures as well as the strengths of these exciting devices and their precious contribution in the field of Neuroscience.
