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This volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
List of contents
Pulsed-field Gel Electrophoresis for Disease Monitoring and Control.- Harmonization of PFGE Profile Analysis by using Bioinformatics Tools: Example of the Listeria monocytogenes European Union Reference Laboratory Network.- PFGE as a Tool to Track Listeria monocytogenes in Food Processing Facilities: Case Studies.- Case Study of the use of Pulsed Field Gel Electrophoresis in the Detection of a Food-borne Outbreak.- Pulsed-Field Gel Electrophoresis for Listeria monocytogenes.- Pulsed-field Gel Electrophoresis (PFGE) for Pathogenic Cronobacter Species.- Pulsed-Field Gel Electrophoresis of Bacillus cereus Group Strains.- Pulsed-Field Gel Electrophoresis of Staphylococcus aureus.- The Use of Pulsed-Field Gel Electrophoresis for Genotyping of Clostridium difficile.- Molecular Subtyping of Clostridium botulinum by Pulsed-Field Gel Electrophoresis.- Pulsed Field Gel Electrophoresis of Yersinia pestis.- Pulsed Field Gel Electrophoresis of Group A streptococci.- Application of Pulsed Field Gel Electrophoresis to Type Campylobacter jejuni.- Pulsed-field Gel Electrophoresis of Pseudomonas aeruginosa.- PFGE for Shiga toxin-producing Escherichia coli O157:H7 (STEC O157) and non-O157 STEC.- Pulsed Field Gel Electrophoresis (PFGE) of Salmonella enterica.- PFGE Protocols to Distinguish Subspecies ofLactococcus lactis.- The use of PFGE Method in Genotyping of Selected Bacteria Species of the Lactobacillus genus.- Pulsed Field Gel Electrophoresis for Leuconostoc mesenteroides and pseudomesenteroides.- Pulsed Field Gel Electrophoresis for Bifidobacterium.- Pulsed Field Gel Electrophoresis for Dairy Propionibacteria.
Summary
This volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
