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Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.
List of contents
Photoinduced Damage Resulting from Fluorescence Imaging of Live Cells.- Modification of Purified Proteins with Photochemical Protection Compounds for High-Resolution Photoactivation of Protein Function In Vitro and In Vivo.- Optochemical Activation of Kinase Function in Live Cells.- Photoswitching of Cell Surface Receptors Using Tethered Ligands.- Photocontrol of AMPA Receptors with a Photochromic Ligand.- Photoconversion of CFP to Study Neuronal Tissue with Electron Microscopy.- Light-Inducible Gene Regulation with Engineered Zinc Finger Proteins.- Manipulation of Plasma Membrane Phosphoinositides using Photoinduced Protein-protein Interactions.- A Roadmap to Applying Optogenetics in Neuroscience.- Salvaging Ruins: Reverting Blind Retinas into Functional Visual Sensors.- Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity.- Structural Basis of Photoswitching in Fluorescent Proteins.- Using Photoactivatable GFP to Track Axonal Transport Kinetics.- In Vivo Cell Tracking Using PhOTO Zebrafish.- In vivo Optogenetics for Light-induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein.- Photoactivatable Fluorescent Proteins for Super-Resolution Microscopy.- pcSOFI as a Smart Label-based Superresolution Microscopy Technique.
Summary
Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.
