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Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery. In Dopamine: Methods and Protocols, experts and key figures within the field provide detailed protocols on leading approaches in the study of dopamine within biological systems. Divided into sections on cellular/biochemical, imaging, genetics, and electrophysiology, this collections includes protocols for bioluminescence and fluorescence imaging, receptor immunoprecipitation and proteomic analysis, creation and characterization of a mouse model of Parkinson's disease, real time measurement of dopamine in the brain, and modeling signal transduction in silico. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and convenient, Dopamine: Methods and Protocols serves as an ideal guide for students and experts alike, as well as for anyone interested in exploring the vast and crucial field of dopamine research.
List of contents
Detection of Cell Surface Dopamine Receptors.- Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells.- Methods of Dopamine Research in Retina Cells.- Capture of D2 Dopamine Receptor Signaling Complexes in Striatal Cells for Mass Spectrometry Proteomic Analysis.- Modeling Spatial Aspects of Intracellular Dopamine Signaling.- A Biophysical Approach for the Study of Dopamine Receptor Oligomerization.- Detection of Receptor Heteromers Involving Dopamine Receptors by the Sequential BRET-FRET (SRET) Technology.- BRET Approaches to Characterize Dopamine and TAAR1 Receptor Pharmacology and Signaling.- Dopaminergic Regulation of Dendritic Calcium: Fast Multi-Site Calcium Imaging.- Functional Analysis of Human D1 and D5 Dopaminergic G Protein-Coupled Receptors: Lessons from Mutagenesis of a Conserved Serine Residue in the Cytosolic End of Transmembrane Region 6.- A Molecular Genetic Approach to Uncovering the Differential Functions of Dopamine D2 Receptor Isoforms.- Genomic Strategies for the Identification of Dopamine Receptor Genes in Zebrafish.- Application of Cell-Specific Isolation to the Study of Dopamine Signaling in Drosophila.- Regulation of Dopamine Transporter Expression by Neuronal Activity.- Monitoring Somatodendritic and Axonal Dopamine Release Using Fast-Scan Cyclic Voltammetry in Brain Slices.- Real-Time Chemical Measurements of Dopamine Release in the Brain.- The MPTP/Probenecid Model of Progressive Parkinson's Disease.
Summary
Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery. In Dopamine: Methods and Protocols, experts and key figures within the field provide detailed protocols on leading approaches in the study of dopamine within biological systems. Divided into sections on cellular/biochemical, imaging, genetics, and electrophysiology, this collections includes protocols for bioluminescence and fluorescence imaging, receptor immunoprecipitation and proteomic analysis, creation and characterization of a mouse model of Parkinson’s disease, real time measurement of dopamine in the brain, and modeling signal transduction in silico. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and convenient, Dopamine: Methods and Protocols serves as an ideal guide for students and experts alike, as well as for anyone interested in exploring the vast and crucial field of dopamine research.
Additional text
From the book reviews:
“Designed for active researchers ranging from students to established scientists, the book is edited by a proteomics researcher working the in the area of dopamine and nicotine receptor protein interaction networks. … this book adds a collection of methods for contemporary techniques that should prove useful to researchers wishing to apply these approaches to their work.” (Beth Levant, Doody’s Book Reviews, May, 2013)
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From the book reviews:
"Designed for active researchers ranging from students to established scientists, the book is edited by a proteomics researcher working the in the area of dopamine and nicotine receptor protein interaction networks. ... this book adds a collection of methods for contemporary techniques that should prove useful to researchers wishing to apply these approaches to their work." (Beth Levant, Doody's Book Reviews, May, 2013)
