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Stem cell mobilization represents a transient increase in the levels of circulating stem and progenitor cells. In Stem Cell Mobilization: Methods in Protocols, expert researchers in the field detail cell mobilization methodology and recent developments in the field for basic and biomedical research community. Specifically clinical hematopoietic progenitor cell mobilization protocols, frontiers in mobilization and analysis of non-hematopoietic progenitors, mesenchymal progenitor cells, monocyte-derived fibroblast progenitors, and very small embryonic like cells. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell Mobilization: Methods and Protocols seeks to aid scientists in the further study of this important stem cell research.
List of contents
Mobilization of Hematopoietic Stem/progenitor Cells: General Principles and Molecular Mechanisms.-Quantifying Hematopoietic Stem and Progenitor Cell Mobilization.-Hematopoietic Stem Cell Mobilization with G-CSF.-Hematopoietic Stem Cell Mobilization with Agents Other Than G-CSF.-Hematopoietic Stem Cell Mobilization: A Clinical Protocol.-Monitoring Blood for CD34+ cells to Determine Timing of Hematopoietic Progenitor Cells (HPC) Apheresis.-Hematopoietic Progenitor Cell Collection.-Managing Apheresis Complications during the Hematopoietic Stem Cell Collection.-Hematopoietic Progenitor Cell Apheresis Processing.-Toxicities of Mobilized Stem Cell Infusion.-Mobilization of Hematopoietic Stem Cells by Depleting Bone Marrow Macrophages.-Combinatorial Stem Cell Mobilization in Animal Models.-Vascular Progenitor Cell Mobilization.-Evaluation of Circulating Endothelial Precursor Cells in Cancer Patients.-Tracking Inflammation-Induced Mobilization of Mesenchymal Stem Cells.-Differentiation of Circulating Monocytes into Fibroblast-like Cells.-Enumeration of Very Small Embryonic Like Stem Cells in Peripheral Blood.-Generation of a Vascular Niche for Studying Stem Cell Homeostasis.-Studying Vascular Progenitor Cells in a Neonatal Mouse Model.-Progenitor Cell Mobilization from Extramedullary Organs.
Summary
Stem cell mobilization represents a transient increase in the levels of circulating stem and progenitor cells.
