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RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets. Written for the highly successful Methods in Molecular Biology(TM) series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Essential and easy to use, RNA Abundance Analysis: Methods and Protocols provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and modified RNAs, which can assist you in your gene expression studies.
List of contents
SuperSAGE: Powerful Serial Analysis of Gene Expression.- Northern Blot Analysis for Expression Profiling of mRNAs and Small RNAs.- Construction of RNA-Seq Libraries from Large and Microscopic Tissues for the Illumina Sequencing Platform.- Strand-Specific RNA-Seq Applied to Malaria Samples.- RNA In Situ Hybridization in Arabidopsis.- Laser Microdissection of Cells and Isolation of High-Quality RNA After Cryosectioning.- Detection and Quantification of Alternative Splicing Variants Using RNA-Seq.- Separating and Analyzing Sulfur-Containing RNAs with Organomercury Gels.- RNAse Mapping and Quantitation of RNA Isoforms.- Detection and Quantification of Viral and Satellite RNAs in Plant Hosts.- In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes.- Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora and Fusarium Using Illumina High Throughput Deep Sequencing.- Isolation and Profiling of Protein-Associated Small RNAs.- New Virus Discovery by Deep Sequencing of Small RNAs.- Global Assembly of Expressed Sequence Tags (ESTs).- Computational Analysis of RNA-Seq.- Identification of MicroRNAs and Natural Antisense Transcript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data.
Summary
RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Essential and easy to use, RNA Abundance Analysis: Methods and Protocols provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and modified RNAs, which can assist you in your gene expression studies.
