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A DNA barcode in its simplest definition is one or more short gene sequences taken from a standardized portion of the genome that is used to identify species through reference to DNA sequence libraries or databases. In DNA Barcodes: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used with DNA barcodes. These methods include the latest information on techniques for generating, applying, and analyzing DNA barcodes across the Tree of Life including animals, fungi, protists, algae, and plants. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, DNA Barcodes: Methods and Protocols aids scientists in continuing to study methods from wet-lab protocols, statistical, and ecological analyses along with guides to future, large-scale collections campaigns.
List of contents
Foreword.-Introduction.-Introduction to Animal DNA Barcoding Protocols.-DNA Barcodes for the Tree of Life - A. Animals - Insects.-Barcoding Methods for Invertebrates.-DNA Barcoding of Amphibians and Reptiles.-DNA Barcoding Fishes.-DNA Barcoding Birds: from Field Collection to Data Analysis.-DNA Barcoding in Mammals.-Methods for DNA Barcoding of Fungi.-Methods for DNA Barcoding Photosynthetic Protists Emphasizing the Macroalgae and Diatoms.-DNA Barcoding Methods for Land Plants.-Field Information Management Systems (FIMS) for DNA Barcoding.-Laboratory Information Management Systems (LIMS) for DNA Barcoding.-DNA Extraction, Preservation and Amplification.-DNA Mini-barcodes.-Next Generation Sequencing: Multiplexing Multiple Barcode Amplicons into Single 454 Run.-The Practical Evaluation of Barcode Efficacy.-Plant DNA Barcodes, Taxonomic Management and Species Discovery in Tropical Forests.-Construction and Analysis of Phylogenetic Trees using DNA Barcode Data.- Phylogenetic Analyses of Ecological Communities Using Barcode Data.-DNA Barcodes: FISH-BOL, a Case Study.-Generating Plant DNA Barcodes for Trees in Long-term Forest Dynamics Plots.- Future Directions.
Summary
A DNA barcode in its simplest definition is one or more short gene sequences taken from a standardized portion of the genome that is used to identify species through reference to DNA sequence libraries or databases. In DNA Barcodes: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used with DNA barcodes. These methods include the latest information on techniques for generating, applying, and analyzing DNA barcodes across the Tree of Life including animals, fungi, protists, algae, and plants. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, DNA Barcodes: Methods and Protocols aids scientists in continuing to study methods from wet-lab protocols, statistical, and ecological analyses along with guides to future, large-scale collections campaigns.
