Read more
The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response.
List of contents
Conventional Murine Gene Targeting.- Production and Characterization of Humanized Rag2-/-Gammac-/- Mice.- Isolation, Culture, and Functional Evaluation of Bone Marrow-Derived Macrophages.- Collecting Resident or Thioglycollate Elicited Peritoneal Macrophages.- Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils.- Assessment of Oxidative Metabolism.- Generation and Culture of Mouse Embryonic Fibroblasts.- Primary Ear Fibroblast Derivation from Mice.- Bone Marrow Derived Dendritic Cells.- Measuring T Cell Function in Innate Immune Models.- Bioassay for the Measurement of Type-I Interferon Activity.- Safe and Effective Mouse Foot-Pad Inoculation.- Delayed Type Hypersensitivity Models in Mice.- Mouse Model of Staphylococcus aureus Skin Infection.- Sepsis Induced by Cecal Ligation and Puncture.- Systemic Infection of Mice with Listeria monocytogenes to Characterize Host Immune Responses.- Mouse Model of Invasive Fungal Infection.- Endotoxin-Induced Uveitis in Rodents.- Bacteria-Mediated Acute Lung Inflammation.- Intranasal Influenza Infection of Mice and Methods to Evaluate Progression and Outcome.- Dextran Sodium Sulfate-Induced Murine Inflammatory Colitis Model.- Bacterial-Mediated Gastrointestinal Inflammation.- Plasmodium berghei ANKA (PbA) Infection of C57BL/6J Mice: A Model of Severe Malaria.- Characterization of HIV-1 Infection in the Humanized Rag2-/-Gammac-/- Mouse Model.
Summary
The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response.
