Read more
In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls
Authoritative and practical, Flower Development: Methods and Protocols is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field.
List of contents
Flower Development in Arabidopsis - There is More to it than Learning your ABCs.- Flower Development in the Asterid Lineage.- Grass Flower Development.- Flower Diversity and Angiosperm Diversification.- Flower Development - Open Questions and Future Directions.- Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors.- Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development.- Genetic and Phenotypic Analyses of Petal Development in Arabidopsis.- Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice.- Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana.- Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis.- Microscopic Analysis of Arabidopsis Ovules.- Scanning Electron Microscopy Analysis of Floral Development.- Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue.- The GUS Reporter System in Flower Development Studies.- A Floral Induction System for the Study of Early Arabidopsis Flower Development.- Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types.- Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers.- Laser-assisted Microdissection Applied to Floral Tissues.- Identification of Arabidopsis Knock-out Lines for Genes of Interest.- Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues.- Misexpression Approaches for the Manipulation of Flower Development.- Next Generation Sequencing Applied to Flower Development: RNA-Seq.- Next-Generation Sequencing Applied to Flower Development: ChIP-Seq.- Live-Imaging of the Arabidopsis Inflorescence Meristem.- Gene Regulatory Network Models for Floral Organ Determination.
Summary
In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls
Authoritative and practical, Flower Development: Methods and Protocols is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field.
