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For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
List of contents
New Opportunities for Immobilization of Enzymes.- Immobilization of Enzymes: A Literature Survey.- Glutaraldehyde Mediated Protein Immobilization.- Immobilization of Enzymes on Mono-and Hetero-Functional Epoxy-Activated Supports.- Stabilization of Enzymes by Multipoint Covalent Immobilization on Supports Activated with Glyoxyl Groups.- Oriented Covalent Immobilization of Enzymes on Heterofunctional-Glyoxyl Supports.- Reversible Covalent Immobilization of Enzymes Via Disulfide Bonds.- Immobilization of Candida rugosa Lipase on Superparamagnetic Fe3O4 Nanoparticles for Biocatalysis in Low Water Media.- Immobilization of Enzymes by Bioaffinity Layering.- Immobilization of Enzymes on Magnetic Beads through Affinity Interactions.- Tips for the Functionalization of Nanoparticles with Antibodies.- Design and Characterization of Functional Nanoparticles for Enhanced Bio-Performance.- Immobilization of Enzymes on Ethynyl-modified Electrodes Via Click Chemistry.- Modification of Carbon Nanotube Electrodes with 1-pyrenebutanoic Acid, Succinimidyl Ester for Enhanced Bioelectrocatalysis.- Enzyme Immobilization by Entrapment within a Gel Network.- Practical Protocols for Lipase Immobilization via Sol-Gel Techniques.- Improving Lipase Activity by Immobilization and Post-Immobilization Strategies.- High Activity Preparations of Lipases and Proteases for Catalysis in Low Water Containing Organic Solvents and Ionic Liquids.- Biomedical Applications of Immobilized Enzymes: An Update.- Immobilization of Whole Cells by Chemical Vapor Deposition of Silica.- Encapsulation of Cells in Alginate Gels.- Microalgal Immobilization Methods.- Therapeutic Applications of Encapsulated Cells.- Whole Cell Entrapment Techniques.
Summary
For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
