Electron Microscopy - Methods and Protocols

This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.

List of contents

Conventional Specimen Preparation Techniques For Transmission Electron Microscopy of Cultured Cells.- Microwave-assisted Processing and Embedding for Transmission Electron Microscopy.- Processing Plant Tissues for Ultrastructural Study.- Staining Sectioned Biological Specimens for Transmission Electron Microscopy: Conventional and En Bloc Stains.- Metal Shadowing for Electron Microscopy.- Freeze Fracture and Freeze Etching.- Conventional Specimen Preparation Techniques For Scanning Electron Microscopy of Biological Specimens.- High-pressure Freezing: Current State and Future Prospects.- Cryo-fixation by Self-pressurized Rapid Freezing.- Cryo-Electron Microscopy of Vitreous Sections.- Negative Staining and Cryo-negative Staining: Applications in Biology and Medicine.- Electron Microscopy of the Microtubule Cytoskeleton Assembly Vitro.- Cryosectioning Fixed and Cryoprotected Biological Material for Immmunocytochemistry.- Analysis of Specificity in Immunoelectron Microscopy.- Cryo-Electron Microscopy of Membrane Proteins.- Tracking DNA and RNA Sequences at High Resolution.- Visualization of DNA and Protein-DNA Complexes with Atomic Force Microscopy.- Biological Applications of Phase-Contrast Electron Microscopy.- Single Particle Cryo-Electron Microscopy And 3-D Reconstruction Of Viruses.- Electron Tomography for Organelles, Cells and Tissues.- Correlative Light and Electron Microscopy: from Live Cell Dynamic to 3D Ultrastructure.- Nanometer-resolution Fluorescence Electron Microscopy (Nano-EM) in Cultured Cells.- Correlative Fluorescence- and Electron Microscopy of Quantum Dot Labeled Proteins on Whole Cells in Liquid.- FIB-SEM Tomography in Biology.- Correlative Light and Electron Microscopy using Immunolabeled Sections.- Correlative 3D Imaging: CLSM and FIB-SEM Tomography Using High-Pressure Frozen, Freeze-Substitued Biological Samples.- Three-dimensional Imaging of Adherent Cells using FIB-SEM and STEM.- X-Ray Microanalysis in the Scanning Electron Microscope.- Application of SEM and EDX in Studying Biomineralization in Plant Tissues.- Freeze Stabilization and Cryopreparation Technique for Visualizing the Water Distribution in Woody Tissues by X-ray Imaging and Cryoscanning Electron Microscopy.- Biological Applications of Energy-Filtered TEM.- Secondary Ion Mass Spectrometry Imaging of Biological Cells and Tissues.- Elemental and Isotopic Imaging of Biological Samples using Nano SIMS.- 3D Chemical Mapping: Application of Scanning Transmission (soft) X-ray Microscopy (STXM) in Combination with Angle-Scan Tomography in Bio-, Geo- and Environmental Science.

Summary

This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.

Additional text

From the book reviews:
“The amount of information in this book is impressive. … The layout of the book is well-organized and the protocols with their accompanying notes are particularly useful and comprehensive. … I would highly recommend this book to any EM laboratory that does not already have the second edition and to larger EM research facilities that are performing the latest correlative microscopy and image analysis techniques.” (Kath White, Microscopy and Microanalysis, Vol. 20 (5), October, 2014)

Report

From the book reviews:
"The amount of information in this book is impressive. ... The layout of the book is well-organized and the protocols with their accompanying notes are particularly useful and comprehensive. ... I would highly recommend this book to any EM laboratory that does not already have the second edition and to larger EM research facilities that are performing the latest correlative microscopy and image analysis techniques." (Kath White, Microscopy and Microanalysis, Vol. 20 (5), October, 2014)

Product details

• Assisted by: Joh Kuo (Editor), John Kuo (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 01.01.2016
• EAN: 9781493960736
• ISBN: 978-1-4939-6073-6
• No. of pages: 799
• Dimensions: 176 mm x 256 mm x 46 mm
• Weight: 1576 g
• Illustrations: XVIII, 799 p. 256 illus., 100 illus. in color.
• Series: Methods in Molecular Biology; Methods in Molecular Biology
• Subject: Natural sciences, medicine, IT, technology > Medicine > Non-clinical medicine