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microRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.
List of contents
Functional Analysis of Exosomal MicroRNA in Cell-Cell Communication Research.- Isolation of Extracellular Nanovesicle MicroRNA from Liver Cancer Cells in Culture .- Methods of Analysis of Dendritic Cell-derived Exosome-shuttle MicroRNA and its Horizontal Propagation Between Dendritic Cells.- Analysis of MicroRNA and Protein Transfer by Exosomes During an Immune Synapse.- Analysis of Viral MicroRNA Exchange via Exosomes In Vitro and In Vivo.- Measurement of Precursor miRNA in Exosomes from Human ESC-Derived Mesenchymal Stem Cells.- Analysis of the Transfer of Circulating microRNA Between Cells Mediated by Gap Junction.- Isolation of Circulating MicroRNA Associated with RNA-binding Protein.- Identification and Analysis of Circulating Exosomal microRNA in Human Body Fluids.- Analyzing the Circulating MicroRNAs in Exosomes/Extracellular Vesicles from Serum or Plasma by qRT-PCR.- Direct Serum Assay for MicroRNA in Cancer Patients.- Analysis of MicroRNA Niches: Techniques to Measure Extracellular MicroRNA and Intracellular MicroRNA In Situ.- Analysis of Circulating MicroRNA by Microarray in Liver Disease .- Isolation of Circulating MicroRNA in Saliva.- Purification of RNA From Milk Whey.- Circulating MicroRNAs in the Cerebrospinal Fluid of Patients with Brain Diseases.- Methods of MicroRNA Quantification in Urinary Sediment.- Circulating microRNA for the Identification of Forensically Relevant Body Fluids.- Identification of Prostate Cancer-associated MicroRNAs in Circulation using a Mouse Model of Disease.- A Combination of Extraction Reagent and DNA Microarray that Allows for the Detection of Global MiRNA Profiles from Serum /Plasma.- Nanopore Single-molecule Detection of Circulating microRNAs.
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Summary
microRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.
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