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Embryonic stem cells and adult stem cells are the two major types of stem cells that have been used for experimental and clinical studies. Embryonic stem cells are totipotent cells that have the capability to differentiate into any type of cell in the body. In Cellular Cardiomyoplasty: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study cellular cardiomyoplasty. Methods and techniques described in this volume use only adult stem cells or adult progenitor cells.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and Practical, Cellular Cardiomyoplasty: Methods and Protocols will benefit the cardiologist, cardiothoracic surgeons, biologist (cell, molecular, or structural), biochemist, and physiologist who are interested in understanding and treating damaged myocardium and failing heart.
List of contents
Cellular Cardiomyoplasty: Its Past, Present and Future.- Skeletal Muscle Stem Cells.- Bone Marrow Stem Cells.- Adipose Tissue Derived Mesenchymal Stem Cells: Isolation, Expansion and Characterization.- Cardiac Side Population Cells and Sca-1-positive Cells.- Two Step Protocol for Isolation and Culture of Cardiospheres.- Generation of Human iPSCs From Human Peripheral Blood Mononuclear Cells using Non-Integrative Sendai Virus in Chemically Defined Conditions.- Identification of Stem Cells after Transplantation.- Methods to Study the Proliferation and Differentiation of Cardiac Side Population (CSP) Cells.- Immune Responses After Mesenchymal Stem Cell Implantation.- Route of Delivery, Cell Retention and Efficiency of Polymeric Microcapsules in Cellular Cardiomyoplasty.- Angiogenic Nanodelivery Systems for Myocardial Therapy.-Bio-Hybrid Tissue Engineering for Cellular Cardiomyoplasty: Future Directions.- Decellularized Whole Heart for Bioartificial Heart.- Clinical Trials of Cardiac Repair with Adult Bone Marrow-Derived Cells.- Clinical Study Using Adipose Derived Mesenchymal-like Stem Cells in Acute Myocardial Infarction and Heart Failure.
Summary
Embryonic stem cells and adult stem cells are the two major types of stem cells that have been used for experimental and clinical studies. Embryonic stem cells are totipotent cells that have the capability to differentiate into any type of cell in the body. In Cellular Cardiomyoplasty: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study cellular cardiomyoplasty. Methods and techniques described in this volume use only adult stem cells or adult progenitor cells.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and Practical, Cellular Cardiomyoplasty: Methods and Protocols will benefit the cardiologist, cardiothoracic surgeons, biologist (cell, molecular, or structural), biochemist, and physiologist who are interested in understanding and treating damaged myocardium and failing heart.
