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Presenting the most up-to-date techniques for the detection, genotyping, and investigation of methicillin-resistant S. aureus (MRSA), this second edition of Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols collects chapters that utilize the power of complete genomic sequences and advanced high-throughput technologies that have pushed this field to its present state. These enable the development of specific and rapid diagnosis methods, the investigation and elucidation of mechanisms of bacterial evolution to antibiotic resistance and pathogenicity, and the identification of novel targets to develop more potent therapeutic and/or preventive agents. Written for the Methods in Molecular Biology series, numerous chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and fully updated, Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols, Second Edition serves as a key reference for researchers attempting to set up a new method to study MRSA or even for technicians and scientists working on other pathogens.
List of contents
Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections.- Community-Associated Methicillin Resistant Staphylococcus aureus Case Studies.- Rapid Methods for Detection of MRSA in Clinical Specimens.- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus (MRSA).- Internal Transcribed Spacer (ITS)-PCR Identification of MRSA.- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Isolates.- Multilocus Sequence Typing (MLST) of Staphylococcus aureus.- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.- Genetic Interruption of Target Genes for Investigation of Virulence Factors.- Molecular Analysis of Staphylococcal Superantigens.- Investigation of Staphylococcus aureus Adhesion and Invasion of Host Cells.- Investigation of Biofilm Formation in Clinical Isolates of Staphylococcus aureus.- Transcriptomic Analysis of Staphylococcus aureus Using Microarray and Advanced Next-Generation RNA-seq Technologies.- Proteomic Approach to Investigate Pathogenicity and Metabolism of Methicillin-Resistant Staphylococcus aureus.- Metobolomic Investigation of Methicillin Resistant Staphylococcus aureus.- Treatment of Infections Due to Resistant Staphylococcus aureus.- Anti-Infective Drug Development for MRSA.- Animal Models in Drug Development for MRSA.
Summary
Presenting the most up-to-date techniques for the detection, genotyping, and investigation of methicillin-resistant S. aureus (MRSA), this second edition of Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols collects chapters that utilize the power of complete genomic sequences and advanced high-throughput technologies that have pushed this field to its present state. These enable the development of specific and rapid diagnosis methods, the investigation and elucidation of mechanisms of bacterial evolution to antibiotic resistance and pathogenicity, and the identification of novel targets to develop more potent therapeutic and/or preventive agents. Written for the Methods in Molecular Biology series, numerous chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and fully updated, Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols, Second Edition serves as a key reference for researchers attempting to set up a new method to study MRSA or even for technicians and scientists working on other pathogens.
