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Galectins: Methods and Protocols is the first book solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that orchestrate adaptive immunity. Given the diverse roles of galectins in a variety of biological systems, studying these glycan binding proteins often requires the assimilation of diverse technical skills to fully appreciate their biological function. Their nearly ubiquitous expression and ability to bind highly modifiable carbohydrate ligands, in addition to a variety of other regulatory proteins, allows these glycan binding proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes. Individual chapters are dedicated to examining salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Galectins: Methods and Protocols seeks to serve both professionals and novices with a useful framework when examining galectin function for many years to come.
List of contents
Evolving Mechanistic Insights into Galectin Functions.- Cloning, Expression and Purification of Galectins for In Vitro Studies.- Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation.- Evaluation of Galectin Binding by Frontal Affinity Chromatography (FAC).- Probing Lectin-Mucin Interactions by Isothermal Titration Microcalorimetry.- Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis.- Evaluation of Galectin Binding by Surface Plasmon Resonance.- Examining Galectin Binding Specificity Using Glycan Microarrays.- Methods for Assessing the Effects of Galectins on Leukocyte Trafficking.- Examination of the Role of Galectins in Plasma Cell Differentiation.- Examination of Galectin-Induced Lattice Formation on Early B-Cell Development.- Detection of Phosophatidylserine Exposure on Leukocytes Following Treatment with Human Galectins.- Examination of Galectins in Phagocytosis.- Assessing the Roles of Galectins in Regulating Dendritic Cell Migration Through Extracellular Matrix and Across Lymphatic Endothelial Cells.- Examination of the Role of Galectins in Intestinal Inflammation.- Study of Galectins in Tumor Immunity: Strategies and Methods.- Galectins in the Regulation of Platelet Biology.- Examination of the Role of Galectins and Galectin Inhibitors in Endothelial Cell Biology.- Regulation of Galectins by Hypoxia and Their Relevance in Angiogenesis: Strategies and Methods.- Examination of the Role of Galectins During In vivo Angiogenesis Using the Chick Chorioallantoic Membrane Assay.- Examination of the Role of Galectins in Cell Migration and Re-Epithelialization of Wounds.- Manipulating Galectin Expression in Zebrafish (Danio rerio).- Examination of Galectin Localization Using Confocal Microscopy.- Examination of the Regulation of Galectin-3 Expression in Cancer.- Evaluation of the Role of Galectins in Parasite Immunity.- Effect of Galectins on Viral Transmission.- Evaluation of the Bactericidal Activity of Galectins.- Examination of the Role of Galectins in Pre-mRNA Splicing.- Analysis of the Intracellular Role of Galectins in Cell Growth and Apoptosis.- Nucleocytoplasmic Shuttling of Galectin-3.
Summary
Galectins: Methods and Protocols is the first book solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that orchestrate adaptive immunity. Given the diverse roles of galectins in a variety of biological systems, studying these glycan binding proteins often requires the assimilation of diverse technical skills to fully appreciate their biological function. Their nearly ubiquitous expression and ability to bind highly modifiable carbohydrate ligands, in addition to a variety of other regulatory proteins, allows these glycan binding proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes. Individual chapters are dedicated to examining salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Galectins: Methods and Protocols seeks to serve both professionals and novices with a useful framework when examining galectin function for many years to come.
