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In Apoptosis and Cancer: Methods and Protocols, Second Edition, expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway. Chapters focus on assays developed to detect its activation not only in vitro but also in vivo, optimized multiplex analysis, medium- to high-throughput screens, and the cellular process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Apoptosis and Cancer: Methods and Protocols, Second Edition aids scientists as a stand-alone resource for the execution and analysis of the described protocols and as a reference for the study and detection of apoptosis within and outside the area of cancer research.
List of contents
Caspase-3 Activation is a Critical Determinant of Genotoxic Stress-induced Apoptosi.- Flow Cytometry Enumeration Of Apoptotic Cancer Cells By Apoptotic Rate.- "Multiplexed Viability, Cytotoxicity and Caspase Activity Assays".- A Multiplexed Method for Kinetic Measurements of Apoptosis and Proliferation using Live Content Imaging.- Detection of End Stage Apoptosis by ApopTag® TUNEL Technique.- Detection and Quantification of Apoptosis in Primary Cells using Taqman® Protein Assay.- Detection of p53 Protein Aggregation in Cancer Cell Lines and Tumor Samples.- Detection of p53 Protein Transcriptional Activity by Chromatin Immunoprecipitation.- Homogeneous, Bioluminescent Proteasome Assays.- Laser Capture Microdissection for Gene Expression Analysis.- Using the Peggy Simple Western System for Fine Needle Aspirate Analysis.- Analysis of Autophagosome Formation Using Lentiviral Biosensors for Live Fluorescent Cellular Imaging.- Optical Imaging of Ovarian Cancer Using HER-2 Affibody Conjugated Nanoparticles.- Measuring Cardiac Autophagic Flux in vitro and in vivo.- PET Imaging for Tyrosine Kinase Inhibitor (TKI) Bio distribution in Mice.
Summary
In Apoptosis and Cancer: Methods and Protocols, Second Edition, expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway. Chapters focus on assays developed to detect its activation not only in vitro but also in vivo, optimized multiplex analysis, medium- to high-throughput screens, and the cellular process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Apoptosis and Cancer: Methods and Protocols, Second Edition aids scientists as a stand-alone resource for the execution and analysis of the described protocols and as a reference for the study and detection of apoptosis within and outside the area of cancer research.
