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This second edition expands upon the previous edition with current, detailed developments in the field and brings together a multi-disciplinary team of leading researchers to provide their latest protocols for clinical proteomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and Practical, Clinical Proteomics: Methods and Protocols, Second Edition presents approaches that will serve as a reliable guide to researchers, including clinicians, chemists, molecular biologists, bioinformaticians and computational, biologists, and investigators working on biomarker development.
List of contents
Biological Sample Collection For Clinical Proteomics - Existing SOPs.- Targeting The Proteome Of Cellular Fractions: Focus on Secretome.- Preparation Of Urinary Exosomes: Methodological Issues For Clinical Proteomics.- Sample Treatment Methods Involving Combinatorial Peptide Ligand Libraries For Improved Proteomes Analyses.- Glycoprotein Enrichment Method Using A Selective Magnetic Nano-Probe Platform (MNP) Functionalized With Lectins.- The Latest Advancements In Proteomic Two-Dimensional Gel Electrophoresis Analysis Applied To Biological Samples.- 2DE Maps In The Discovery Of Human Autoimmune Kidney Diseases: The Case of Membranous Glomerulonephritis.- MALDI-Imaging Mass Spectrometry on Tissues.- Laser Capture Microdissection Of Fluorescently Labeled Amyloid Plaques From Alzheimer's Disease Brain Tissue For Mass Spectrometric Analysis.- Urine Sample Preparation And Fractionation For Global Proteome Profiling By LC-MS.- Methods In Capillary Electrophoresis Coupled To Mass Spectrometry For The Identification Of Clinical Proteomic/Peptidomic Biomarkers In Biofluids.- Quantification Of Proteins In Urine Samples Using Targeted Mass Spectrometry Methods.- Statistical Issues In The Design And Planning Of Proteomic Profiling Experiments.- Integrating Proteomics Profiling Data Sets - A Network Perspective.- The European Medicines Agency Experience With Biomarker Qualification.
Summary
This second edition expands upon the previous edition with current, detailed developments in the field and brings together a multi-disciplinary team of leading researchers to provide their latest protocols for clinical proteomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and Practical, Clinical Proteomics: Methods and Protocols, Second Edition presents approaches that will serve as a reliable guide to researchers, including clinicians, chemists, molecular biologists, bioinformaticians and computational, biologists, and investigators working on biomarker development.
