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Plant Isoprenoids: Methods and Protocols is a collection of detailed techniques that will be a useful tool for a wide range of plant biologists, as well as for scientists of other fields interested in plant isoprenoids. Isoprenoids are an incredibly diverse family and they participate in a large variety of processes. Divided into four convenient sections to better cover strategic areas in plant isoprenoid research, topics include measurement of core enzyme activities involved in the production of isoprenoid precursors, targeted analysis of major groups of isoprenoid metabolites, isoprenoid profiling in specialized organs such as trichomes and oil glands as well as genetic, pharmacological and bioinformatic tools that are particularly useful for plant molecular biologists. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Plant Isoprenoids: Methods and Protocols will serve as an excellent reference material that can be adapted to develop customized methods for different needs making the world of plant isoprenoids more accessible for all researchers.
List of contents
Plant Isoprenoids: A General Overview.- Measuring the Activity of 1-Deoxy-D-Xylulose 5-Phosphate Synthase, the First Enzyme in the MEP Pathway, in Plant Extracts.- Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants.- Farnesyl Diphosphate Synthase Assay.- Metabolite Profiling of Plastidial Deoxyxylulose-5-Phosphate Pathway Intermediates by Liquid Chromatography and Mass Spectrometry.- Analysis of Carotenoids and Tocopherols in Plant Matrices and Assessment of Their In Vitro Antioxidant Capacity.- Simultaneous Analyses of Oxidized and Reduced Forms of Photosynthetic Quinones by High-Performance Liquid Chromatography.- Determination of Sterol Lipids in Plant Tissues by Gas Chromatography and Q-TOF Mass Spectrometry.- Analysis of Plant Polyisoprenoids.- Analysis of Diterpenes and Triterpenes from Plant Foliage and Roots.- Gas Chromatography Mass-Spectrometry Method for Determination of Biogenic Volatile Organic Compounds Emitted by Plants.- Analysis of Steroidal Alkaloids and Saponins in Solanaceae Plant Extracts using UPLC-qTOF Mass Spectrometry.- Isoprenoid and Metabolite Profiling of Plant Trichomes.- Sample Preparation for Single Cell Transcriptomics - Essential Oil Glands in Citrus Fruit Peel as an Example.- Prenylquinone Profiling in Whole Leaves and Chloroplast Subfractions.- Confocal Laser Scanning Microscopy Detection of Chlorophylls and Carotenoids in Chloroplasts and Chromoplasts of Tomato Fruit.- Heterologous Expression of Triterpene Biosynthetic Genes in Yeast and Subsequent Metabolite Identification Through GC-MS.- High Throughput Testing of Terpenoid Biosynthesis Candidate Genes Using Transient Expression in Nicotiana benthamiana.- Heterologous Stable Expression of Terpenoid Biosynthetic Genes Using the Moss Physcomitrella patens.- Quantification of Plant Resistance to Isoprenoid Biosynthesis Inhibitors.- A Flexible Protocol for Targeted Gene Co-Expression Network Analysis.
Summary
Plant Isoprenoids: Methods and Protocols is a collection of detailed techniques that will be a useful tool for a wide range of plant biologists, as well as for scientists of other fields interested in plant isoprenoids. Isoprenoids are an incredibly diverse family and they participate in a large variety of processes. Divided into four convenient sections to better cover strategic areas in plant isoprenoid research, topics include measurement of core enzyme activities involved in the production of isoprenoid precursors, targeted analysis of major groups of isoprenoid metabolites, isoprenoid profiling in specialized organs such as trichomes and oil glands as well as genetic, pharmacological and bioinformatic tools that are particularly useful for plant molecular biologists. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Plant Isoprenoids: Methods and Protocols will serve as an excellent reference material that can be adapted to develop customized methods for different needs making the world of plant isoprenoids more accessible for all researchers.
