Read more
Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.
List of contents
An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae.- Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae.- Tools for Genetic Engineering of the Yeast Hansenula polymorpha.- Molecular Tools and Protocols for Engineering the Acid Tolerant Yeast Zygosaccharomyces bailii as a Potential Cell Factory.- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.- Methods for Efficient High Throughput Screening of Protein Expression in Recombinant Pichia pastoris Strains.- Synthetic RNA Switches for Yeast Metabolic Engineering: Screening Recombinant Enzyme Libraries.- Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology.- Genetic Engineering of Industrial Saccharomyces cerevisiae Strains Using a Selection/Counter-Selection Approach.- Evolutionary Engineering of Yeast.- Determination of a Dynamic Feeding Strategy for Recombinant Pichia pastoris Strains.- Yeast Metabolomics: Sample Preparation for a GC/MS-Based Analysis.- 13C-Based Metabolic Flux Analysis in Yeast: The Pichia pastoris Case.- Pathway Activity Profiling (PAPi): A Tool for Metabolic Pathway Analysis.- QTL Mapping by Pooled-Segregant Whole Genome Sequencing in Yeast.- Genome Scale Metabolic Models of Yeast, Methods for Their Reconstruction and Other Applications.- Model-Guided Identification of Gene Deletion Targets for Metabolic Engineering in Saccharomyces cerevisiae.- Patents: A Tool to Bring Innovation from the Lab Bench to the Marketplace.
Summary
Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.
