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Regulatory B Cells: Methods and Protocols present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Regulatory B Cells: Methods and Protocols serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology.
List of contents
Purification and Immunophenotypic Characterization of Murine MZ and T2-MZP Cells.- Purification and Immune-Phenotyping of B-1 Cells from Body Cavities of Mice.- Purification and Immunophenotypic Characterization of Murine B10 B Cells.- Purification and Immunophenotypic Characterization of Human B Cells with Regulatory Functions.- IL-10 Detection in Murine B Cells: Pros and Cons of the Different Techniques.- TGF-beta Detection and Measurement in Murine B Cells: Pros and Cons of the Different Techniques.- Characterization and Activity of Fas Ligand Producing CD5+ B Cells.- Utilization of a Lentiviral System for the Generation of B Cells with Regulatory Properties.- Ex Vivo Generation of Murine IL-10-Producing B Cells by Fusokines.- Toll-Like Receptor Ligation for the Induction of Regulatory B Cells.- The Generation of Regulatory B Cells by Helminth Parasites.- Use of Co-Cultures for the Study of Cellular Interactions Influencing B Cell Regulatory Functions.- Regulatory B Cells in Experimental Mouse Models of Arthritis.- Regulatory B Cells in Mouse Models of Systemic Lupus Erythematosus (SLE).- Regulatory B Cells in Allergic Airways Disease and Asthma.- Regulatory B Cells in Mouse Models of Intestinal Inflammation.- Regulatory B Cells in Experimental Autoimmune Encephalomyelitis (EAE).- Regulatory B Cells, Helminths and Multiple Sclerosis.- Generation and Identification of Tumor-Evoked Regulatory B Cells.
Summary
Regulatory B Cells: Methods and Protocols present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Regulatory B Cells: Methods and Protocols serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology.
