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How can I use my HPLC/UHPLC equipment in an optimal way, where are the limitations of the technique? These questions are discussed in detail in the sequel of the successful "HPLC Expert" in twelve chapters written by experts in the respective fields. The topics encompass - complementary to the first volume - typical HPLC users' problems and questions such as gradient optimization and hyphenated techniques (LC-MS). An important key aspect of the book is UHPLC: For which analytical problem is it essential, what should be considered? Besides presentation of latest developments directly from the main manufacturers, also UHPLC users and independent service engineers impart their knowledge. Consistent with the target groups, the level is advanced, but the emphasis is on practical applications.
List of contents
Introduction: When should I use my UHPLC as a UHPLC?
PART 1: HARDWARE AND SOFTWARE, SEPARATION MODES, MATERIALS
Modern HPLC/UHPLC equipment
Requirements for the different parts of HPLC/UHPLC equipment
The column oven - a simple matter?
The problem of the extra column dispersion in an HPLC/UHPLC equipment
The gradient: Requirements, optimal operation, tricks and pitfalls
Hardware requirements for LC coupling with different mass spectrometers
2D-Chromatography - potentials and limitations
Selecting the right column materials in HPLC and UHPLC
Qality by Design in HPLC Method Development
PART 2: USE CASES AND TRENDS
What should the software be able to do, so that the hardware can be used to its full potential?
Aspects of modern HPLC - a user´s experience report
Hints and recommendations for optimal use of HPLC equipment
Using UHPLC to solve the compound separation problem
Equipment review (Agilent, Shimadzu, ThermoScientific)
About the author
Stavros Kromidas worked as Sales Manager for Waters Chromatography following his PhD thesis completed in 1983. From 1989-2001 he was Managing Director of Novia GmbH, a consultant company for analytical chemistry and is an independent consultant since then. He has worked in the area of HPLC since 1978 and has given lectures and training courses since 1984.
Summary
How can I use my HPLC/UHPLC equipment in an optimal way, where are the limitations of the technique? These questions are discussed in detail in the sequel of the successful "HPLC Expert" in twelve chapters written by experts in the respective fields. The topics encompass - complementary to the first volume - typical HPLC users' problems and questions such as gradient optimization and hyphenated techniques (LC-MS). An important key aspect of the book is UHPLC: For which analytical problem is it essential, what should be considered? Besides presentation of latest developments directly from the main manufacturers, also UHPLC users and independent service engineers impart their knowledge. Consistent with the target groups, the level is advanced, but the emphasis is on practical applications.
