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Hox Genes: Methods and Protocols explores techniques and methodologies which arose from or were successfully applied to the study of Hox genes and Hox proteins, at the intersection of experimental embryology, genetics, biochemistry, physiology, evolutionary biology and other life sciences. This detailed volume begins with a section on discovery and functional analysis of Hox genes and then it continues onward to discuss mode of action and biomedical applications of Hox proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Expert and practical, Hox Genes: Methods and Protocols serves as an ideal guide to researchers striving to move forward in this dynamic and exciting area of study.
List of contents
Discovery and Classification of Homeobox Genes in Animal Genomes.- How to Study Hox Gene Expression and Function in Mammalian Oocytes and Early Embryos.- Genetic Lineage Tracing Analysis of Anterior Hox Expressing Cells.- A Genetic Strategy to Obtain P-Gal4 Elements in the Drosophila Hox Genes.- Hox Complex Analysis Through BAC Recombineering.- The Genetics of Murine Hox Loci: Tamere, String and Panthere to Engineer Chromosome Variants.- Topological Organization of Drosophila Hox Genes Using DNA Fluorescent In Situ Hybridization.- Mining the Cis-Regulatory Elements of Hox Clusters.- Functional Analysis of Hox Genes in Zebrafish.- Transgenesis in Non-Model Organisms: The Case of Parhyale.- Tissue Specific RNA Isolation in Drosophila Embryos: A Strategy to Analyze Context Dependent Transcriptome Landscapes Using FACS.- Hox Transcriptomics in Drosophila Embryos.- Measuring Hox-DNA Binding by Electrophoretic Mobility Shift Analysis.- Chromatin Immunoprecipitation and Chromatin Immunoprecipitation with Massively Parallel Sequencing on Mouse Embryonic Tissue.- ChIP for Hox Proteins from Drosophila Imaginal Discs.- SELEX-seq: A Method for Characterizing the Complete Repertoire of Binding Site Preferences for Transcription Factor Complexes.- DamID as an Approach to Studying Long Distance Chromatin Interactions.- cgChIP: A Cell Type and Gene Specific Method for Chromatin Analysis.- Bimolecular Fluorescence Complementation (BiFC) in Live Drosophila Embryos.- Hox Protein Interactions: Screening and Network Building.- Rational Drug Repurposing Using sscMap Analysis in a HOX-TALE Model of Leukemia.
Summary
Hox Genes: Methods and Protocols explores techniques and methodologies which arose from or were successfully applied to the study of Hox genes and Hox proteins, at the intersection of experimental embryology, genetics, biochemistry, physiology, evolutionary biology and other life sciences. This detailed volume begins with a section on discovery and functional analysis of Hox genes and then it continues onward to discuss mode of action and biomedical applications of Hox proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Expert and practical, Hox Genes: Methods and Protocols serves as an ideal guide to researchers striving to move forward in this dynamic and exciting area of study.
