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This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a "plant chromosome vector" system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination. The second approach is "genome-editing," which makes it possible to introduce mutations into any of the genes of DNA that we wish to change. In addition, this book also covers other related techniques used to accelerate progress in plant chromosome and genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Chromosome and Genomic Engineering in Plants: Methods and Protocols provides a comprehensive source of protocols and other necessary information to anyone interested in this field of study.
List of contents
Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences.- Method for Biolistic Site-Specific Integration in Rice and Tobacco Catalyzed by Bxb1 Integrase.- Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase Mediated Gene Stacking.- Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants.- One-Step Generation of Chromosomal Rearrangements in Rice.- Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana.- Gametocidal Systems for Dissecting Wheat Chromosomes.- CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases.- Targeted Mutagenesis in Rice Using and TALENs and the CRISPR/Cas9 System.- Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination.- Development of Genome Engineering Tools fromPlant-Specific PPR Proteins Using Animal Cultured Cells.- Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes.- Image Analysis of DNA Fiber and Nucleus in Plants.- Detection of Transgenes on DNA Fibers.- Three-Dimensional, Live-Cell Imaging of Chromatin Dynamics in Plant Nuclei Using Chromatin Tagging Systems.- Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes.- Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements.
Summary
This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a “plant chromosome vector” system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination. The second approach is “genome-editing,” which makes it possible to introduce mutations into any of the genes of DNA that we wish to change. In addition, this book also covers other related techniques used to accelerate progress in plant chromosome and genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Chromosome and Genomic Engineering in Plants: Methods and Protocols provides a comprehensive source of protocols and other necessary information to anyone interested in this field of study.
