Stem cells - In vitro neurogenesis of human and rat bone marrow derived mesenchymal stem cells

This study aimed to investigate the ability of mesenchymal stem cells derived from bone marrow of human and rat to transdifferentiate into functional neural like cells. A study was made on two different models (human and rat) using two different techniques (direct plating, and density gradient method) respectively. Immunophenotypic analysis demonstrated that human and rat BM-MSCs at passage three uniformly positive for CD44 sub-population. However, MSCs were always found to be negative for heamatopoietic specific markers CD34. To explore the possibility of BM-MSCs in vitro transdifferentiation, the expanded MSCs from third passage were allowed to reach 80% confluence and induced to differentiate into neuron like cells by an enhanced three-step protocol. The identity of the NLCs were illustrated by the analysis of morphology using immunocytochemistry.

About the author

Sarah M. S. Al-Sawahha EDUCATION:April. 2015 - Baghdad University, Candidate for master degree in Biology\ animal physiology.Jun 2012, - Baghdad University,Candidate for Bachelor in Biology.EMPLOYMENT HISTORY:Sep.2015Lab technician in stem cell culture in Alzahrawy center for stem cells therapy.

Product details

• Authors: Sara Al-Sawalha, Sarah Al-Sawalha, Abdul M Hammadi, Abdul Majeed Hammadi, Jabba Yanzeel, Jabbar Yanzeel
• Publisher: LAP Lambert Academic Publishing
• Languages: English, German
• Product format: Paperback / Softback
• Released: 01.01.2016
• EAN: 9783659858338
• ISBN: 978-3-659-85833-8
• No. of pages: 144
• Dimensions: 150 mm x 220 mm x 8 mm
• Weight: 233 g
• Subject: Natural sciences, medicine, IT, technology > Biology > Zoology