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This volume aims to provide a range of methods and protocols for studying tumor angiogenesis in vitro and in vivo to reflect advances in the field. The chapters in this book cover topics such as: morphological aspects of tumor angiogenesis, aortic ring assay and its use for the study of tumor angiogenesis, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Tumor Angiogenesis Assays: Methods and Protocols is a valuable resource for anyone interested in tumor angiogenesis assay research.
List of contents
The Discovery of Tumor Angiogenesis Factors: An Historical Overview.- Morphological Aspects of Tumor Angiogenesis.- Exosomes in Tumor Angiogenesis.- Co-Culture Assays for Endothelial Cells: Mural Cells Interactions.- Characterization of Cancer Stem Cells.- The Aortic Ring Assay and its Use for the Study of Tumor Angiogenesis.- Surface Plasmon Resonance Analysis of Heparin-Binding Angiogenic Growth Factors.- An Ex Vivo Tissue Culture Model for Anti-Angiogenic Drug Testing.- The Chorioallantoic Membrane of the Chick Embryo to Assess Tumor Formation and Metastasis.- Transgenic Zebrafish.- High Resolution Intravital Microscopy of Tumor Angiogenesis.- Subcutaneous Murine Xenograft Models: A Critical Tool for Studying Human Tumor Growth and Angiogenesis In Vivo.- Orthotopic Models of Ovarian Cancer.- The Rip1Tag2 Transgenic Mouse Model.- Tumor Blood Vessel Visualization.- Uncovering Metabolic Effects of Anti-Angiogenic Therapy in Tumors by Induced Metabolic Bioluminescence Imaging.- Quantitation of Tumor Angiogenesis In Vitro: An All-In-One Angiogenesis Assay.
Summary
This volume aims to provide a range of methods and protocols for studying tumor angiogenesis in vitro and in vivo to reflect advances in the field. The chapters in this book cover topics such as: morphological aspects of tumor angiogenesis, aortic ring assay and its use for the study of tumor angiogenesis, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Tumor Angiogenesis Assays: Methods and Protocols is a valuable resource for anyone interested in tumor angiogenesis assay research.
