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This volume is the result of an explosion ofmolecular-based research on Cilia, which began with the discovery of theuniversality of intraflagellar transport (IFT) and ciliary genomics/proteomics.The chapters in this book cover topics such as: high resolution imaging andfunctional characterization of sensory and primary cilia in mammalian cells andzebrafish, methods to study ciliary-mediated chemoresponse in Paramecium,and methods to study centrosomes and cilia in C. elegans and Drosophila.Written in the highly successful Methods in Molecular Biology series format,chapters include introduction to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Cilia:Methods and Protocols is broad and covers motile, sensory, and primarycells. It is a valuable resource to anyone interested in entering the field ofciliary biology using model organisms, including flagellate algae, ciliates,planaria, nematodes, insects, zebrafish, and mammalian cells.
List of contents
Methods for Studying Ciliary Import Mechanisms.- Targeting of ASH Domain-Containing Proteins tothe Centrosome.- Morphological and Functional Characterizationof the Ciliary Pocket by Electron and Fluorescence Microscopy.- Methods to Study Interactions between Ciliogenesisand Autophagy.- Recombinant Reconstitution and Purification ofthe IFT B Core Complex from Chlamydomonasreinhardtii.- Methods for Studying Movement of Moleculeswithin Cilia.- A FRAP-Based Method for Monitoring Molecular Transportin Ciliary Photoreceptor Cells In Vivo.- Kymographic Analysis of Transport in an Individual Neuronal Sensory Cilium in Caenorhabditis elegans.- Visualization and Manipulation of Cilia and IntraciliaryCalcium in the Zebrafish Left-Right Organizer.- Methods for Studying Ciliary-Mediated Chemoresponsein Paramecium.- STEDand STORM Superresolution Imaging of Primary Cilia.- CLEM Methods forStudying Primary Cilia.- Methods for Visualization of Neuronal Cilia.- Methods to Study Centrosomes and Cilia in Drosophila.- Analysis of Axonemal Assembly during Ciliary Regenerationin Chlamydomonas.- Planaria as aModel System for the Analysis of Ciliary Assembly and Motility.
Summary
This volume is the result of an explosion of
molecular-based research on Cilia, which began with the discovery of the
universality of intraflagellar transport (IFT) and ciliary genomics/proteomics.
The chapters in this book cover topics such as: high resolution imaging and
functional characterization of sensory and primary cilia in mammalian cells and
zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium,
and methods to study centrosomes and cilia in C. elegans and Drosophila.
Written in the highly successful Methods in Molecular Biology series format,
chapters include introduction to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Cilia:
Methods and Protocols is broad and covers motile, sensory, and primary
cells. It is a valuable resource to anyone interested in entering the field of
ciliary biology using model organisms, including flagellate algae, ciliates,
planaria, nematodes, insects, zebrafish, and mammalian cells.
