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A comprehensive collection of perspectives by experts in mycobacterial molecular biology
Mycobacterium tuberculosis causes one in four avoidable deaths in the developing world and kills more adults than malaria, AIDS, and all tropical diseases combined. Tuberculosis was named a global health emergency by the World Health Organization, a distinction no other disease has received. Although the study of mycobacterial genetics has expanded dramatically, with new investigations into mycobacterial growth, replication, metabolism, physiology, drug susceptibility, and virulence, most of the problems in tuberculosis control that existed in 2000 remain today.
Advances in our understanding of mycobacterial genetics have been reflected in exciting recent developments. New diagnostic approaches can identify drug resistance within a few hours, promising new drugs are progressing through the pipeline and into the clinic, and a range of newly developed vaccines are being evaluated. It is an exciting time as the fruits of 30 years of intensive genetic investigation are finally beginning to emerge.
Written by leading experts in the field, Molecular Genetics of Mycobacteria, Second Edition,
* Discusses key areas of current research in mycobacterial genetics
* Explains the genetics of the physiology, metabolism, and drug sensitivities of M. tuberculosis
* Presents genetic approaches for manipulating M. tuberculosis
This book is an invaluable resource for anyone interested in the molecular genetics and molecular biology of mycobacteria.
List of contents
Contributors
Preface
I. Genomes, Genomics, and Genetic Exchange
1. Gene Transfer In Mycobacterium Tuberculosis: Shuttle Phasmids to Enlightenment
William R. Jacobs, Jr.
2. Mycobacterial Pathogenomics and Evolution
Daria Bottai, Timothy P. Stinear, Philip Supply, and Roland Brosch
3. BCG Vaccines
Vanessa Tran, Jun Liu, and Marcel A. Behr
4. Distributive Conjugal Transfer: New Insights into Horizontal Gene Transfer and Genetic Exchange in Mycobacteria
Keith M. Derbyshire and Todd A. Gray
5. Molecular Genetics of Mycobacteriophages
Graham F. Hatfull
6. Genetics of Phage Lysis
Madalena Pimentel
II. Gene Expression and Regulation
7. Sigma Factors: Key Molecules In Mycobacterium Tuberculosis Physiology and Virulence
Riccardo Manganelli
8. Transcription Factor Binding Site Mapping Using ChIP-Seq
Suma Jaini, Anna Lyubetskaya, Antonio Gomes, Matthew Peterson, Sang Tae Park, Sahadevan Raman, Gary Schoolnik, and James Galagan
9. Noncoding RNA in Mycobacteria
Kristine B. Arnvig, Teresa Cortes, and Douglas B. Young
10. Two-Component Regulatory Systems of Mycobacteria
Tanya Parish
11. Regulated Expression Systems for Mycobacteria and Their Applications
Dirk Schnappinger and Sabine Ehrt
III. The Mycobacterial Proteome
12. Mycobacterium Tuberculosis In The Proteomics Era
Martin Gengenbacher, Jeppe Mouritsen, Olga T. Schubert, Ruedi Aebersold, and Stefan H. E. Kaufmann
13. Structural Annotation of the Mycobacterium tuberculosis Proteome
Nagasuma Chandra, Sankaran Sandhya, and Praveen Anand
14. Cyclic AMP Signaling in Mycobacteria
Gwendowlyn S. Knapp and Kathleen A. McDonough
IV. Metabolism
15. The Physiology and Genetics Of Oxidative Stress In Mycobacteria
Bridgette M. Cumming, Dirk Lamprecht, Ryan M. Wells, Vikram Saini, James H. Mazorodze, and Adrie J. C. Steyn
16. Metabolomics of Central Carbon Metabolism in Mycobacterium tuberculosis
Anthony D. Baughn and Kyu Y. Rhee
17. Mycobacterial Lipidomics
Emilie Layre, Reem Al-Mubarak, John T. Belisle, and D. Branch Moody
18. Genetics of Mycobacterial Trehalose Metabolism
Rainer Kalscheuer and Hendrik Koliwer-Brandl
19. Metallobiology of Tuberculosis
G. Marcela Rodriguez and Olivier Neyrolles
20. Energetics of Respiration and Oxidative Phosphorylation in Mycobacteria
Gregory M. Cook, Kiel Hards, Catherine Vilchèze, Travis Hartman, and Michael Berney
V. Genetics of Drug Resistance
21. Molecular Basis of Drug Resistance In Mycobacterium Tuberculosis
Keira A. Cohen, William R. Bishai, and Alexander S. Pym
22. Resistance to Isoniazid and Ethionamide in Mycobacterium tuberculosis: Genes, Mutations, and Causalities
Catherine Vilchèze and William R. Jacobs, Jr.
23. The Molecular Genetics of Fluoroquinolone Resistance in Mycobacterium tuberculosis
Claudine Mayer and Howard Takiff
24. Mechanisms of Pyrazinamide Action and Resistance
Ying Zhang, Wanliang Shi, Wenhong Zhang, and Denis Mitchison
25. Genetic Strategies for Identifying New Drug Targets
Andrej Trauner, Christopher M. Sassetti, and Eric J. Rubin
VI. Genetics of Membrane and Cell Wall Biosynthesis
26. Genetics of Peptidoglycan Biosynthesis
Martin S. Pavelka, Jr., Sebabrata Mahapatra, and Dean C. Crick
27. Genetics of Mycobacterial Arabinogalactan and Lipoarabinomannan Assembly
Monika Jankute, Shipra Grover, Helen L. Birch, and Gurdyal S. Besra
28. Genetics of Capsular Polysaccharides and Cell Envelope (Glyco)lipids
Mamadou Daffé, Dean C. Crick, and Mary Jackson
29. The Molecular Genetics of Mycolic Acid Bios
Summary
A comprehensive collection of perspectives by experts in mycobacterial molecular biology
Mycobacterium tuberculosis causes one in four avoidable deaths in the developing world and kills more adults than malaria, AIDS, and all tropical diseases combined. Tuberculosis was named a global health emergency by the World Health Organization, a distinction no other disease has received. Although the study of mycobacterial genetics has expanded dramatically, with new investigations into mycobacterial growth, replication, metabolism, physiology, drug susceptibility, and virulence, most of the problems in tuberculosis control that existed in 2000 remain today.
Advances in our understanding of mycobacterial genetics have been reflected in exciting recent developments. New diagnostic approaches can identify drug resistance within a few hours, promising new drugs are progressing through the pipeline and into the clinic, and a range of newly developed vaccines are being evaluated. It is an exciting time as the fruits of 30 years of intensive genetic investigation are finally beginning to emerge.
Written by leading experts in the field, Molecular Genetics of Mycobacteria, Second Edition,
* Discusses key areas of current research in mycobacterial genetics
* Explains the genetics of the physiology, metabolism, and drug sensitivities of M. tuberculosis
* Presents genetic approaches for manipulating M. tuberculosis
This book is an invaluable resource for anyone interested in the molecular genetics and molecular biology of mycobacteria.
