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This volume covers arange of methods used in plant cytogenetics, beginning with basic analysis ofchromosomes and visualizing gene locations, to manipulating and dissectingchromosomes, and then focusing on less understood features of chromosomes suchas recombination initiation sites and epigenomic marks. The methods describedin Plant Cytogenetics: Methods and Protocols build on each other andprovide, those new to the field, with a comprehensive platform to support theirresearch endeavours, while also introducing advanced techniques to experiencedresearchers. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Cuttingedge and thorough, Plant Cytogenetics: Methods and Protocols, is avaluable resource for anyone who is interested in the diverse and wonderfullycomplex field of cytogenetics.
List of contents
C-Banding of Plant Chromosomes.- Chromosome Painting by GISH and Multi-ColorFISH.- Fluorescent In Situ Hybridization on Extended Chromatin Fibers forHigh-Resolution Analysis of Plant Chromosomes.- Tyramide Signal Amplification: FluorescenceIn Situ Hybridization for IdentifyingHomologous Chromosomes.- Localizationof Low-Copy DNA Sequences on Mitotic Chromosomes by FISH.- Immunolabelingand In Situ Labelling of IsolatedPlant Interphase Nuclei.- Manipulationof Homologous Chromosome Recombination in Wheat.- DissectingPlant Chromosomes by the Use of Ionizing Radiation.- OpticalNano-Mapping and Analysis of Plant Genomes.- FlowSorting Plant Chromosomes.- Constructionof BAC Libraries from Flow-Sorted Chromosomes.- TheChromosome Microdissection and Microcloning Technique.- Immunolocalizationon Whole Anther Chromosome Spreads for Male Meiosis.- MappingRecombination Initiation Sites Using Chromatin Immunoprecipitation.- ChromatinImmunoprecipitation to Study Plant Epigenome.
About the author
Shahryar Kianian Research Leader USDA-ARS Cereal Disease Laboratory University of Minnesota St. Paul, MN 55108
Email: Shahryar.Kianian@ars.usda.gov
Penny M Avoles Kianian Alderman Hall, St. Paul, MN 55108
Email:P.Kianian@gmail.com
Summary
This volume covers a
range of methods used in plant cytogenetics, beginning with basic analysis of
chromosomes and visualizing gene locations, to manipulating and dissecting
chromosomes, and then focusing on less understood features of chromosomes such
as recombination initiation sites and epigenomic marks. The methods described
in Plant Cytogenetics: Methods and Protocols build on each other and
provide, those new to the field, with a comprehensive platform to support their
research endeavours, while also introducing advanced techniques to experienced
researchers. Written in the highly successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting
edge and thorough, Plant Cytogenetics: Methods and Protocols, is a
valuable resource for anyone who is interested in the diverse and wonderfully
complex field of cytogenetics.
