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Informationen zum Autor Laurens Welles was born in 1983 in The Hague, The Netherlands. In 2002 he started his BSc study Life Science and Technology at Delft University of Technology and Leiden University. He specialized in Cell Factory during his MSc study at Delft in 2006, and conducted his MSc research project on the Enhanced Biological Phosphorus Removal process at the University of Tokyo followed by an internship focused on bioremediation at Kurita Water Industries in Japan. In 2010, he was appointed as PhD fellow in the SALINE project at UNESCO-IHE and consortium partners KWR Watercycle Research Institute, Delft University of Technology, University of Cape Town, The Hong Kong University of Science and Technology, The Higher Polytechnic Institute "José Antonio Echeverría" and Birzeit University. The main objective of the project was to assess the feasibility of using saline water directly for non-potable purposes such as flushing toilets in urban areas to mitigate fresh water consumption. Within this project, the aim of his PhD research was to get a better understanding of the salinity effects on Enhanced Biological Phosphorus Removal in activated sludge systems to support the design and development of operational guidelines for WWTPs treating saline wastewater generated when saline water is used directly for non-potable purposes. Currently Laurens is working as a postdoctoral researcher for UNESCO-IHE and Delft University of Technology in The Netherlands. At UNESCO-IHE he assists in the management of a project, "Stimulating local innovation in sanitation for Sub-Saharan Africa and South East Asia." At the Delft University of Technology he conducts research on the anaerobic metabolism of Phosphate-Accumulating Organisms (PAO) and Glycogen-Accumulating Organisms (GAO), the diversity of this metabolism among different PAO and GAO clades and implications for nutrient removal and recovery systems. Klappentext The Enhanced Biological Phosphorus Removal (EBPR) process is a biological process for efficient phosphate removal from wastewaters through intracellular storage of polyphosphate by Phosphate-Accumulating Organisms (PAO). This thesis is dedicated to two different aspects of the EBPR process. The first part of the thesis focuses on the functional diversity of PAO clades and its influence on process performance. The second part describes the salinity effects on the metabolism of PAO and their competitors, the Glycogen-Accumulating Organisms (GAO) that do not contribute to phosphorus removal. The potential implications of sudden saline shocks in wastewater treatment systems not regularly exposed to salinity are also discussed. Zusammenfassung The Enhanced Biological Phosphorus Removal (EBPR) process is a biological process for efficient phosphate removal from wastewaters through intracellular storage of polyphosphate by Phosphate-Accumulating Organisms (PAO) and subsequent removal through wastage of excess sludge. Although many studies have demonstrated the existence of different PAO clades, the functional differences among these clades and potential implications for the process performance remained unclear. Furthermore, the salinity effects on the EBPR process had not been properly investigated, which is necessary to assess its applicability for the treatment of saline wastewaters. The first part of the thesis focuses on the functional diversity among PAO clades. It demonstrates significant functional differences in the main characteristics of the anaerobic metabolism of two different PAO clades and provides fundamental insight in the metabolic response of PAO to different influent P/C ratios. In addition, it shows how these functional differences provide competitive advantages to specific PAO clades in a selection study and discusses their potential implications on process performance, in particular for combined biological and ch...
