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This volume provides researchers with up-to-date protocols and takes aclose look at current research and promising applications. Optogenetics:Methods and Protocols contains a collection of recently developed technicalprotocols on optogenetic applications in neuroscience, brain mapping, treatmentof neurological disorders, and restoration of visual function. Severalintroductory and discussion chapters offer a wide overview about sources anddiversity of optogenetic tools, design strategies, and potential applicationsin other fields like plant research. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Cutting edge and thorough, Optogenetic: Methods and Protocols, deliversan interdisciplinary view of optogenetic applications and its potential todevelop as a valuable tool for basic research, as well as biomedical andbiotechnological applications.
List of contents
Optogenetics:Basic Concepts and Their Development.- NaturalResources for Optogenetic Tools.- Algal Photobiology: A Rich Source ofUnusual Light Sensitive Proteins for Synthetic Biology and Optogenetics.- Reversible Photoregulation of geneExpression and Translation.- Controlling Protein Activity andDegradation Using Blue-Light.- Photo-Control of Protein Function UsingPhotoactive Yellow Protein.- A Fluorometric Activity Assay forLight-Regulated Cyclic-Nucleotide-Monophosphate Actuators.- Optogenetic Control of Pancreatic Islets.- Optogenetics in Plants: Red/Far-Red LightControl of Gene Expression.- Perfect Channelrhodopsins: A User Guide.- Optogenetics in Drosophila Neuroscience.- Optogenetic Control of Mammalian IonChannels with Chemical Photoswitches.- Optogenetic Modulation of LocomotorActivity on Free Behaving Rats.- Combines Optogenetic and ChemogeneticControl of Neurons.- Intracranial Injection ofan OptogeneticsViral Vector Followed by Optical Cannula Implantation for Neural Stimulation inRat Brain Cortex.- An Optomized Calcium-Phosphate TransfectionMethod for Characterizing Genetically Encoded Tools in Primary Neurons.- Optogenetic Approaches for Mesoscopic BrainMapping.- Optogenetic Tools for Confined Stimulationin Deep Brain Structures.- Remote Patterning of Transgene ExpressionUsing Near Infrared-Responsive Plasmonic Hydrogels.- Optogenetic Light Crafting Tools for theControl of Cardiac Arrhythmias.- Inscribing Optocal Excitability toNon-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in PrimaryCardiac Fibroblasts.- Optogenetic Engineering of AtrialCardiomyocytes.- A Multi-Channel Recording System withOptical Stimulation for Closed-Loop Optogenetic Experiments.- Optogenetic Control of Fibroblast GrowthFactor Receptor Signaling.- Protein Inactivation by OptogeneticTrapping in Living Cells.- Optogenetic Manipulation of SelectiveNeural Activity in Free-Moving DrosophilaAdults.- Guidelines for Photoreceptor Engineering.
Summary
This volume provides researchers with up-to-date protocols and takes a
close look at current research and promising applications. Optogenetics:
Methods and Protocols contains a collection of recently developed technical
protocols on optogenetic applications in neuroscience, brain mapping, treatment
of neurological disorders, and restoration of visual function. Several
introductory and discussion chapters offer a wide overview about sources and
diversity of optogenetic tools, design strategies, and potential applications
in other fields like plant research. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Cutting edge and thorough, Optogenetic: Methods and Protocols, delivers
an interdisciplinary view of optogenetic applications and its potential to
develop as a valuable tool for basic research, as well as biomedical and
biotechnological applications.
