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This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.
List of contents
Animal Coronaviruses: A Brief Introduction.- Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissues.- Virus Neutralization Assay for Turkey Coronavirus Infection.- Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli.- Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen.- Reverse Genetics of Avian Coronavirus Infectious Bronchitis Virus.- Vaccinia Virus-Based Reverse Genetics for Feline Coronaviruses.- Real-Time RT-PCR Detection of Equine Coronavirus.- Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction.- Real-Time Reverse Transcription Polymerase Chain Reaction for Rapid Detection of Transmissible Gastroenteritis Virus.- An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes.- A Multiplex Polymerase Chain Reaction forDifferential Detection of Turkey Coronavirus from Chicken Infectious Bronchitis Virus and Bovine Coronavirus.- Real-Time Reverse Transcription-polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues.- PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene.- Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis.- Genotype-Specific Detection of Ferret Coronavirus by Conventional and Real-Time Reverse Transcription Polymerase Chain Reaction.- Molecular Characterization of Canine Coronavirus.- Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus by Illumina MiSeq Platform.- Next Generation Sequencing for Porcine Coronaviruses.
Summary
This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.
Additional text
“This book is unique in presenting current technologies available for detection of animal coronaviruses and research especially for diagnostic methodologies. The intention is to provide specific protocols and methods for individual coronaviruses, both conventional approaches and state-of-the art technologies. … The primary audience is diagnosticians, interns, and residents who work in the laboratory, but graduate students and researchers will benefit as well. … The book is essential for researchers and diagnosticians in veterinary virology.” (Dongwan Yoo, Doody's Book Reviews, June, 2016)
Report
"This book is unique in presenting current technologies available for detection of animal coronaviruses and research especially for diagnostic methodologies. The intention is to provide specific protocols and methods for individual coronaviruses, both conventional approaches and state-of-the art technologies. ... The primary audience is diagnosticians, interns, and residents who work in the laboratory, but graduate students and researchers will benefit as well. ... The book is essential for researchers and diagnosticians in veterinary virology." (Dongwan Yoo, Doody's Book Reviews, June, 2016)
