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This volume presents protocols on yeast cytokinesis that will help Molecular and Cellular Biology researchers in the use of these microorganisms to approach the study of general or specific key questions in cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Yeast Cytokinesis: Methods and Protocols provides practical and step-by-step detailed protocols useful for a wide audience ranging from experienced researchers to beginners in the use of yeasts.
List of contents
Time-lapse Fluorescence Microscopy Of Budding Yeast Cells.- Real-time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells.- Fluorescence Recovery After Photo-bleaching (FRAP) and Fluorescence Loss in Photo-bleaching (FLIP) Experiments to Study Protein Dynamics during Budding Yeast Cell Division.- High-Speed Super Resolution Imaging of Live Fission Yeast Cells.- Monitoring Chitin Deposition during Septum Assembly in Budding Yeast.- Imaging Septum Formation by Fluorescence Microscopy.- Visualization of Cytokinesis Events in Budding Yeast by Transmission Electron Microscopy.- Visualization of Fission Yeast Cells By Transmission Electron Microscopy.- Characterization of Septin Ultrastructure in Budding Yeast Using Electron Tomography.- Isolation of Cytokinetic Actomyosin Rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe.- Measurements of Myosin-II motor Activity during Cytokinesis In Fission Yeast.- In Vitro Biochemical Characterization of Cytokinesis Actin Binding Proteins.- Characterization of Cytokinetic F-BARs and other Membrane Binding Proteins.- Analysis of Three-Dimensional Structures of Exocyst Components.- Analysis of Rho-GTPase Activity during Budding-yeast Cytokinesis.- Detection of Phosphorylation Status of Cytokinetic Components.- Studying Protein-Protein Interactions in Budding Yeast Using Co-Immunoprecipitation.- Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening Of Suppressor Genes.- Synchronization of the Budding Yeast Saccharomyces cerevisiae.- Fission Yeast Cell Cycle Synchronization Methods.- A Review of Fluorescent Proteins for Use in Yeast.- Visualisation and Image Analysis of Yeast Cells.- Toolbox for Protein Structure Prediction.- From Structure to Function. A Comprehensive Compendium of Tools to Unveil Protein Domains and Understand their Role in Cytokinesis.
Summary
This volume presents protocols on yeast cytokinesis that will help Molecular and Cellular Biology researchers in the use of these microorganisms to approach the study of general or specific key questions in cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Yeast Cytokinesis: Methods and Protocols provides practical and step-by-step detailed protocols useful for a wide audience ranging from experienced researchers to beginners in the use of yeasts.
