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Divided into three parts this volume summarizes the most important areas of Cell-Penetrating Peptides (CPP) research . Part one briefly presents the historical background of CPP studies and the classifications of the available CPPs, and then summarizes the approaches for prediction of novel CPPs. Part two mainly describes the methods for studies of "naked" CPPs, that is, CPPs without conjugated cargos. Last but not least part three presents a representative and brief summary of functionality issues of CPPs, both in vitro and in vivo. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Concise and easy-to-use, Cell-Penetrating Peptides: Methods and Protocols, Second Edition hopes to raise relevant questions for further development.
List of contents
Classes of Cell-Penetrating Peptides.- Penetratin Story: An Overview.- Prediction of Cell-Penetrating Peptides.- Computer-Aided Virtual Screening and Designing Of Cell-Penetrating Peptides.- Investigating Membrane Interactions and Structures Of Cpps.- Determining the Effects Of Membrane-Interacting Peptides on Membrane Integrity.- Study of CPP Mechanisms By Mass-Spectrometry.- Methods to Study the Role of the Glycocalyx in the Uptake of Cell Penetrating Peptides.- Toxicity, Immunogenicity, Uptake and Kinetics Methods for Cpps.- Unravelling the Mechanisms Of Peptide-Mediated Delivery of Nucleic Acids Using Electron Microscopy.- SCARA Involvement in the Uptake of Nanoparticles Formed by Cell Penetrating Peptides.- Protein Mimicry and the Design of Bioactive Cell Penetrating Peptides.- Pepducins and Other Lipidated Peptides as Mechanistic Probes and Therapeutics.- Identification and Characterization of Homing Peptides Using In Vivo Peptide Phage Display.- Antiviral and Antimicrobial Applications Of Cell Penetrating Peptides.- Visualising Actin Architectures in Cells Incubated with Cell Penetrating Peptides.- Cell-Penetrating Peptides as Carriers For Transepithelial Drug Delivery In Vitro.- A Pathway Toward Tumor Cell Selective Cpps?.- Characterization of Cellular Internalization Pathways for CPP-Mediated Oligonucleotide Delivery Pepfects and Nickfects.- In Vitro Assays to Assess Exon Skipping in Duchenne Muscular Dystrophy.- Applications of Apob LDLR Binding Domain Approach for the Development of CNS Penetrating Peptides for Alzheimer's Disease.-Utilization of Cpps for In Vivo Delivery of Bioactive Cargo - The Effect Of Cargo Type, CPP-Cargo Association Method and Formulation.- Application of Cpps for Brain Delivery.- Intracellular Delivery of Nanoparticles with Cell Penetrating Peptides.- Multifunctional Oligoaminoamides for The Receptor-Specific Delivery of Therapeutic RNA.- Cell Penetrating Peptides for Chemical Biological Studies.- Experiences with CPP-Based Self Assembling Peptide Systems for Topical Delivery of Botulinum Toxin.- Applications of Cpps in Gene Modulation of Plants.- DNA Transfer Into Animal Cells Using Stearyled CPP Based Transfection Reagent.- Live Cell Genomics: Cell-Specific Transcriptome Capture in Live Tissues and Cells.- Live Cell Genomics: RNA Exon-Specific RNA Binding Protein Isolation.
Summary
Divided into three parts this volume summarizes the most important areas of Cell-Penetrating Peptides (CPP) research . Part one briefly presents the historical background of CPP studies and the classifications of the available CPPs, and then summarizes the approaches for prediction of novel CPPs. Part two mainly describes the methods for studies of “naked” CPPs, that is, CPPs without conjugated cargos. Last but not least part three presents a representative and brief summary of functionality issues of CPPs, both
in vitro
and
in vivo
. As a volume in the highly successful
Methods in Molecular Biology
series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Concise and easy-to-use
, Cell-Penetrating Peptides: Methods and Protocols, Second Edition
hopes to raise relevant questions for further development.
