Fundamentals of Scientific Research - An Introductory Laboratory Manual

Informationen zum Autor Marcy A. Kelly is Professor and Assistant Chair of the Department of Biology at Pace University in New York, NY, USA. Pryce L. Haddix is an Associate Professor of Biology at Auburn University in Montgomery, AL, USA. Klappentext The Fundamentals of Scientific Research: An Introductory Laboratory Manual is a laboratory manual geared towards first semester undergraduates enrolled in general biology courses focusing on cell biology. This laboratory curriculum centers on studying a single organism throughout the entire semester - Serratia marcescens, or S. marcescens, a bacterium unique in its production of the red pigment prodigiosin.The manual separates the laboratory course into two separate modules. The first module familiarizes students with the organism and lab equipment by performing growth curves, Lowry protein assays, quantifying prodigiosin and ATP production, and by performing complementation studies to understand the biochemical pathway responsible for prodigiosin production. Students learn to use Microsoft Excel to prepare and present data in graphical format, and how to calculate their data into meaningful numbers that can be compared across experiments. The second module requires that the students employ UV mutagenesis to generate hyper-pigmented mutants of S. marcescens for further characterization. Students use experimental data and protocols learned in the first module to help them develop their own hypotheses, experimental protocols, and to analyze their own data.Before each lab, students are required to answer questions designed to probe their understanding of required pre-laboratory reading materials. Questions also guide the students through the development of hypotheses and predictions. Following each laboratory, students then answer a series of post-laboratory questions to guide them through the presentation and analysis of their data, and how to place their data into the context of primary literature. Students are also asked to review their initial hypotheses and predictions to determine if their conclusions are supportive. A formal laboratory report is also to be completed after each module, in a format similar to that of primary scientific literature.The Fundamentals of Scientific Research: An Introductory Laboratory Manual is an invaluable resource to undergraduates majoring in the life sciences. Zusammenfassung The Fundamentals of Scientific Research: An Introductory Laboratory Manual is a laboratory manual geared towards first semester undergraduates enrolled in general biology courses focusing on cell biology. Inhaltsverzeichnis Preface xi Acknowledgments xv About the Companion Website xvii Introduction xix Module 1 Working with and Learning About Common Laboratory Techniques and Equipment 1 Exercise 1A Using Common Laboratory Tools to Evaluate Measurements Pre-laboratory Thinking Questions 3 Exercise 1B Using Common Laboratory Tools to Evaluate Measurements 4 Exercise 1C Using Common Laboratory Tools to Evaluate Measurements Post-laboratory Thinking Questions 18 Exercise 2A Using Microscopy to Evaluate Cell Size and Complexity Pre-laboratory Thinking Questions 19 Exercise 2B Using Microscopy to Evaluate Cell Size and Complexity 20 Exercise 2C Using Microscopy to Evaluate Cell Size and Complexity Post-laboratory Thinking Questions 32 Exercise 3A The Bacterial Growth Curve Pre-laboratory Thinking Questions 35 Exercise 3B The Bacterial Growth Curve 36 Exercise 3C The Bacterial Growth Curve Post-laboratory Thinking Questions 55 Module 2 Working with and Learning About Serratia marcescens in the Laboratory 57 Exercise 4A Protein Concentration Versus Growth Stage Pre-laboratory Thinking Questions 59 Exercise 4B Protein Concentration Versus Growth Stage 60 Exercise 4C Protein C...

List of contents

Preface xi
 
Acknowledgments xv
 
About the Companion Website xvii
 
Introduction xix
 
Module 1 Working with and Learning About Common Laboratory Techniques and Equipment 1
 
Exercise 1A Using Common Laboratory Tools to Evaluate Measurements Pre-laboratory Thinking Questions 3
 
Exercise 1B Using Common Laboratory Tools to Evaluate Measurements 4
 
Exercise 1C Using Common Laboratory Tools to Evaluate Measurements Post-laboratory Thinking Questions 18
 
Exercise 2A Using Microscopy to Evaluate Cell Size and Complexity Pre-laboratory Thinking Questions 19
 
Exercise 2B Using Microscopy to Evaluate Cell Size and Complexity 20
 
Exercise 2C Using Microscopy to Evaluate Cell Size and Complexity Post-laboratory Thinking Questions 32
 
Exercise 3A The Bacterial Growth Curve Pre-laboratory Thinking Questions 35
 
Exercise 3B The Bacterial Growth Curve 36
 
Exercise 3C The Bacterial Growth Curve Post-laboratory Thinking Questions 55
 
Module 2 Working with and Learning About Serratia marcescens in the Laboratory 57
 
Exercise 4A Protein Concentration Versus Growth Stage Pre-laboratory Thinking Questions 59
 
Exercise 4B Protein Concentration Versus Growth Stage 60
 
Exercise 4C Protein Concentration Versus Growth Stage Post-laboratory Thinking Questions 69
 
Exercise 5A Measuring Prodigiosin Pre-laboratory Thinking Questions 71
 
Exercise 5B Measuring Prodigiosin 72
 
Exercise 5C Measuring Prodigiosin Post-laboratory Thinking Questions 84
 
Exercise 6A Conditions Affecting the Growth of and Prodigiosin Production by Serratia marcescens Pre-laboratory Thinking Questions 87
 
Exercise 6B Conditions Affecting the Growth of and Prodigiosin Production by S. marcescens 91
 
Exercise 6C Formal Laboratory Report Describing the Conditions Affecting the Growth of and Prodigiosin Production by S. marcescens 95
 
Exercise 7A Biochemistry of Prodigiosin Production Pre-laboratory Thinking Questions 101
 
Exercise 7B Biochemistry of Prodigiosin Production 102
 
Exercise 7C Biochemistry of Prodigiosin Production Post-laboratory Thinking Questions 112
 
Exercise 8A The Probability Basis for Mutation Rate Calculation: A Dice?]Roll Exercise Pre-laboratory Thinking Questions 113
 
Exercise 8B The Probability Basis for Mutation Rate Calculation: A Dice?]Roll Exercise 114
 
Exercise 8C The Probability Basis for Mutation Rate Calculation: A Dice?]Roll Exercise Post-laboratory Thinking Questions 120
 
Exercise 9A Understanding Evolution by the Generation of UV Light?]Induced Prodigiosin Mutants Pre-laboratory Thinking Questions 121
 
Exercise 9B Understanding Evolution by the Generation of UV Light?]Induced Prodigiosin Mutants 122
 
Exercise 9C Understanding Evolution by the Generation of UV Light?]Induced Prodigiosin Mutants Post-laboratory Thinking Questions 131
 
Exercise 10A Understanding the Energy Spilling Properties of Prodigiosin Pre-laboratory Thinking Questions 137
 
Exercise 10B Understanding the Energy Spilling Properties of Prodigiosin 139
 
Exercise 10C Understanding the Energy Spilling Properties of Prodigiosin Post-laboratory Thinking Questions 146
 
Module 3 Initial Characterization of Novel Serratia marcescens Prodigiosin Mutants 147
 
Exercise 11A Prodigiosin Mutant Study Part 1 Pre-laboratory Thinking Questions 149
 
Exercise 11B Prodigiosin Mutant Study Part 1 150
 
Exercise 11C Prodigiosin Mutant Study Part 1 Post-laboratory Thinking Questions 153
 
Exercise 12A Prodigiosin Mutant Study Part 2 Pre-laboratory Thinking Questions 155
 
Exercise 12B Prodigiosin Mutant Study Part 2 156
 

Product details

• Authors: Pryce L Haddix, Pryce L. Haddix, Ma Kelly, Marcy Kelly, Marcy A Kelly, Marcy A. Kelly, Marcy A. Haddix Kelly, Kelly Marcy A., Marcy A. Kelly
• Publisher: Wiley, John and Sons Ltd
• Languages: English
• Product format: Paperback / Softback
• Released: 26.02.2016
• EAN: 9781118867846
• ISBN: 978-1-118-86784-6
• No. of pages: 208
• Subjects: Natural sciences, medicine, IT, technology > Biology > General, dictionaries; Mikrobiologie, Medizin, Molekularbiologie, Life Sciences, ZELLBIOLOGIE, Biowissenschaften, Cell Biology, Medical Science, Medizinische Zellbiologie, Medical Cell Biology, Molekulare Mikrobiologie, Molecular Microbiology