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Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. As part of the highly successful Methods in Molecular Biology(TM) series, the chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Yeast Functional Genomics and Proteomics: Methods and Protocols is an ideal reference for both yeast researchers and those who wish to use yeast as a model system for the further study of functional genomics and proteomics.
List of contents
Comparative Genome Hybridization on Tiling Microarrays to Detect Aneuploidies in Yeast.- Identification of Transcription Factor Targets by Phenotypic Activation and Microarray Expression Profiling in Yeast.- SGAM: An Array-Based Approach for High-Resolution Genetic Mapping in Saccharomyces cerevisiae.- Reporter-Based Synthetic Genetic Array Analysis: A Functional Genomics Approach for Investigating the Cell Cycle in Saccharomyces cerevisiae.- The Fidgety Yeast: Focus on High-Resolution Live Yeast Cell Microscopy.- A Genomic Approach to Yeast Chronological Aging.- Chemogenomic Approaches to Elucidation of Gene Function and Genetic Pathways.- Identification of Inhibitors of Chromatin Modifying Enzymes Using the Yeast Phenotypic Screens.- Exploiting Yeast Genetics to Inform Therapeutic Strategies for Huntington's Disease.- Global Proteomic Analysis of Saccharomyces cerevisiae Identifies Molecular Pathways of Histone Modifications.- Systematic Characterization of the Protein Interaction Network and Protein Complexes in Saccharomyces cerevisiae Using Tandem Affinity Purification and Mass Spectrometry.- Protein Microarrays.- Analysis of Protein-Protein Interactions Using Array-Based Yeast Two-Hybrid Screens.- Analysis of Membrane Protein Complexes Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System.- Computational Analysis of the Yeast Proteome: Understanding and Exploiting Functional Specificity in Genomic Data.
Summary
Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. As part of the highly successful Methods in Molecular Biology™ series, the chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Yeast Functional Genomics and Proteomics: Methods and Protocols is an ideal reference for both yeast researchers and those who wish to use yeast as a model system for the further study of functional genomics and proteomics.
Additional text
From the reviews:
“This volume takes a snapshot of the questions which can be asked with a genome … . A major value of this book may be in helping to overcome the fear factor. Most of the approaches are feasible in a moderately resourced laboratory, and as the tools for S. cerevisiae spread to other organisms, so will the potential usefulness of this collection.” (John Armstrong, Microbiology Today, January, 2010)
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From the reviews: "This volume takes a snapshot of the questions which can be asked with a genome ... . A major value of this book may be in helping to overcome the fear factor. Most of the approaches are feasible in a moderately resourced laboratory, and as the tools for S. cerevisiae spread to other organisms, so will the potential usefulness of this collection." (John Armstrong, Microbiology Today, January, 2010)
